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January 1996
Volume:20 , Issue Number:1
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Internet On-Ramp
Using Newsgroups: Virtual Conferences on Specialized Topics
Robert M. Horton
 
Short Technical Reports
Bicistronic Vector for the Creation of Stable Mammalian Cell Lines that Predisposes All Antibiotic-Resistant Cells to Express Recombinant Protein
S. Rees, J. Coote, J. Stables, S. Goodson, S. Harris, and M.G. Lee
Full Text (PDF)
Enhanced Cytochemical Detection of Viral Proteins and RNAs Using Double-Sided Labeling and Light Microscopy
Xin Shun Ding, Shelly A. Carter, and Richard S. Nelson
Full Text (PDF)
Cloning Strategies Using a Third Irrelevant Enzyme Site (TIES) to Overcome Certain Cloning Problems
Ming Zeng, Bing-Ren Huang, and Jean-Numa Lapeyre
Full Text (PDF)
Cloning of Size-Selected Human Immunoglobulin Heavy-Chain Rearrangements from Third Complementarity-Determining Region Fingerprint Profiles
Frank M. Raaphorst, Joseph Tami, and Ignacio Sanz
Full Text (PDF)
Gene Transfer into Subcultured Endometrial Cells Using Lipofection
I. Lascombe, P. Mougin, C. Vuillermoz, G.L. Adessi, and M. Jouvenot
Full Text (PDF)
Harvest Protocol to Reduce Variability of Soluble Enzyme Yield From Cultured Cells
Jean N. Buskin, DeeAnn L. Gregory, William A. LaFramboise, and Stephen D. Hauschka
Full Text (PDF)
 
Research Reports
Expression of a Bifunctional Chimeric Protein A-Vargula hilgendorfii Luciferase in Mammalian Cells
Y. Maeda, H. Ueda, T. Hara, J. Kazami, G. Kawano, E. Suzuki, and T. Nagamune
Full Text (PDF)
Synthesis of a New Substrate for Detection of lacZ Gene Expression in Live Drosophila Embryos
Jonathan S. Minden
Full Text (PDF)
Construction of a Restriction Map and Gene Map of the Lettuce Chloroplast Small Single-Copy Region Using Southern Cross-Hybridization
Keith R. Mitchelson
Full Text (PDF)
 
Benchmarks
Device for the Simultaneous Screening of Bacteriophage Lambda Clones
Lluis Espinosa and Estanis Navarro
Full Text (PDF)
Preparation of T-Overhang Vectors with High PCR Product Cloning Efficiency
Noureddine Hadjeb and Gerald A. Berkowitz
Full Text (PDF)
Simple 5' Extension Method Allowing Transcript Discrimination
Colette Breda, Joumana El Turk, and Robert Esnault
Full Text (PDF)
Transfer Technique for Minimizing Waste of Sonified Adjuvant Emulsions
Edward G. Spack and Deby Toavs
Full Text (PDF)
Removal of Albumin from Multiple Human Serum Samples
Kalpana Rengarajan, Marc D. de Smet, and Barbara Wiggert
Full Text (PDF)
Measurement of 8-OHdG in DNA by HPLC/ECD: The Importance of DNA Purity
George M. Laws and Stephen P. Adams
Full Text (PDF)
Transient Gene Transfer into Myotubes Following Differentiation in Culture
R. Brooks Robey, Haruhiko Osawa, Richard L. Printz, and Daryl K. Granner
Full Text (PDF)
Preparation of Electro-Competent E. coli Using Salt-free Growth Medium
Rakesh C. Sharma and Robert T. Schimke
Full Text (PDF)
Preparation of Electro-Competent E. coli Using Salt-free Growth Medium
Rakesh C. Sharma and Robert T. Schimke
Full Text (PDF)
Measurement of Human Growth Hormone Using a Chemiluminescence Assay and a "Glow" Luminometer
Peter T. Guidon, Jr., Rudolph Peter, and Elise Kumar
Full Text (PDF)
Simple Method for Adapting DNA Fragments and PCR Products to All of the Commonly Used Restriction Sites
T.C. Tsang, D.T. Harris, E.T. Akporiaye, S.F. Schluter, G.T. Bowden, and E.M. Hersh
Full Text (PDF)
Chemiluminescent Detection of Unique Sequences on Chromosomes After On-Slide PCR
Hongyan Xie and Deryl L. Troyer
Full Text (PDF)
PCR-TIES (Third Irrelevant Enzyme Site)-Mediated Gene Fusion Method
Ming Zeng and Jean-Numa Lapeyre
Full Text (PDF)
 
Product Application Focus
Vectors for Expression and Secretion of FLAG Epitope-Tagged Proteins in Mammalian Cells
Richard G. Chubet and Bill L. Brizzard
Full Text (PDF)