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April 2006 Articles
Volume:40 , Issue Number:4
Cover Image:
Human bone marrow endothelial-1 (HBME-1) cells. Mitochondria (red) were labeled in living cells with MitoTracker® Red; a biotinylated phage display peptide (L13) was localized with streptavidin-conjugated Alexa Fluor™ 488 (green); and nuclei (blue) were labeled with DRAQ5™. Cells were imaged using a Zeiss 510 NLO two-photon confocal microscope with Coherent Mira 900F titanium sapphire laser, argon laser, He/Ne laser, and 633 nm HeNe laser at 40x magnification. Image courtesy of Manisha Malik and Robert A. Sikes, Laboratory for Cancer Ontogeny and Therapeutics, Department of Biological Sciences, University of Delaware, Newark, DE.
BioSpotlight
BioSpotlight
BioTechniques, Vol. 40, No. 4, April 2006, pp. 411–413
doi  10.2144/06404BS01
 
Citations
Citations
BioTechniques, Vol. 40, No. 4, April 2006, pp. 415–417
doi  10.2144/06404CI01
 
Scientists
Profile of Jennifer Lippincott-Schwartz, Ph.D.
Laura Bonetta
BioTechniques, Vol. 40, No. 4, April 2006, pp. 419
doi  10.2144/06404SP01
 
About the Authors
The Muraguchi Laboratory
BioTechniques, Vol. 40, No. 4, April 2006, p. 543
doi  10.2144/06404AA02
The Departments of Cell Biology and Cellular Assay Systems
BioTechniques, Vol. 40, No. 4, April 2006, p. 544
doi  10.2144/06404AA01
 
Review
Advances in fluorescent tracking of nucleic acids in living cell
Roeland W. Dirks, Hans J. Tanke
BioTechniques, Vol. 40, No. 4, April 2006, pp. 489–496
doi  10.2144/000112121
 
Short Technical Reports
Amplification and analysis of cDNA generated from a single cell by 5′-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells
Tatsuhiko Ozawa, Hiroyuki Kishi, Atsushi Muraguchi
BioTechniques, Vol. 40, No. 4, April 2006, pp. 469–478
doi  10.2144/000112123
Direct quantification of gene expression in homogenates of formalin-fixed, paraffin-embedded tissues
Wen Yang, Botoul Maqsodi, Yunqing Ma, Son Bui, Kimberly L. Crawford, Gary K. McMaster, Frank Witney, Yuling Luo
BioTechniques, Vol. 40, No. 4, April 2006, pp. 481–486
doi  10.2144/000112133
 
Benchmarks
One-step high-throughput assay for quantitative detection of β-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cells
Faustino Vidal-Aroca, Michele Giannattasio, Elisa Brunelli, Alessandro Vezzoli, Paolo Plevani, Marco Muzi-Falconi, Giovanni Bertoni
BioTechniques, Vol. 40, No. 4, April 2006, pp. 433–440
doi  10.2144/000112145
Evaluation method of ordinary flatbed scanners for quantitative density analysis
Satoshi Nishizuka, Newell R. Washburn, Peter J. Munson
BioTechniques, Vol. 40, No. 4, April 2006, pp. 442–448
doi  10.2144/000112144
Device for handling electron microscopy grids
Mika Lännenpää, Juhani E. Syväoja
BioTechniques, Vol. 40, No. 4, April 2006, pp. 450–452
doi  10.2144/000112147
Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood
Piotr Chomczynski, Michal Rymaszewski
BioTechniques, Vol. 40, No. 4, April 2006, pp. 454–458
doi  10.2144/000112149
Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells
John P. Russell, David W. Chang, Anna Tretiakova, Malla Padidam
BioTechniques, Vol. 40, No. 4, April 2006, pp. 460–464
doi  10.2144/000112150
 
Webwatch
WebWatch
Kevin Ahern
BioTechniques, Vol. 40, No. 4, April 2006, p. 421
doi  10.2144/06404WW01
 
Techniques Essay
Protein Microarrays
Chien-Sheng Chen, Heng Zhu
BioTechniques, Vol. 40, No. 4, April 2006, pp. 423–429
doi  10.2144/06404TE01
 
Research Report
Detection of high levels of recombination generated during PCR amplification of RNA templates
Wayne Yu, Karl J. Rusterholtz, Amber T. Krummel, Niles Lehman
BioTechniques, Vol. 40, No. 4, April 2006, pp. 499–507
doi  10.2144/000112124
Use of a zinc fluorophore to measure labile pools of zinc in body fluids and cell-conditioned media
Peter Zalewski, Ai Truong-Tran, Stephen Lincoln, David Ward, Anu Shankar, Peter Coyle, Lata Jayaram, Andrew Copley, Dion Grosser, Chiara Murgia, Carol Lang, Richard Ruffin
BioTechniques, Vol. 40, No. 4, April 2008, pp. 509–520
doi  10.2144/06404RR02
Cell adhesion profiling using extracellular matrix protein microarrays
Cornelia Kuschel, Heiko Steuer, Andreas N. Maurer, Britta Kanzok, Reinout Stoop, Brigitte Angres
BioTechniques, Vol. 40, No. 4, April 2006, pp. 523–531
doi  10.2144/000112134
 

 
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