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The Stagljar lab uses a combination of molecular, cellular, and proteome approaches to study the function of many yeast and human membrane proteins, as well as proteins involved in the maintenance of genome stability in humans. Current projects within the lab focus on four critical areas. First, the lab uses the membrane yeast two-hybrid technology to identify proteins associated with numerous yeast and human integral membrane proteins of medical importance, as well as to understand the roles of these newly identified proteins in the related processes in yeast and man. Second, the lab is developing a novel yeast-based protein interaction assay based on the large-scale pull-down analyses of the endogenously tagged yeast open reading frames (ORFs) labeled with two different epitope tags. Third, the lab uses the baker's yeast Saccharomyces cerevisiae as a whole-cell system to identify small molecule inhibitors of the defined ORFs from the opportunistic human pathogenic bacterium, Pseudomonas aeruginosa PAO-1. Lastly, the lab investigates the role of the human RecQ family members during DNA replication, repair, and recombination processes.
biochemistry.utoronto.ca/stagljar/index.html
In our review article, we give a comprehensive overview of significant studies in functional genomics and proteomics related to the budding yeast S. cerevisiae. The review encompasses a summary of research using genome-wide collections of yeast deletion strains, including studies of drug-induced haploinsufficency, and large-scale studies in protein localization and protein interactions. Particularly, studies of protein interactions are discussed in the context of research using TAP-tagging/mass spectrometry, protein microarrays, and various versions of the yeast two-hybrid assay. Lastly, we emphasize the strengths, shortcomings, and promise of these yeast-based methodologies.
Yeast-based functional genomics and proteomics technologies: the first 15 years and beyond, p. 625.
