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April 2007 Articles
Volume:42
, Issue Number:4
Cover Image:
Coronal thin section of mouse brain labeled with antibody against heavy chain neurofilament subunits followed by secondary antibody conjugated to Alexa Fluor® 488 (green). Glial fibrillary acidic protein (GFAP), which is strongly and specifically expressed in various astroglia and neural stem cells, was labeled with anti-GFAP antibody and visualized with secondary antibody conjugated to Alexa Fluor 568 (red). Nuclear DNA was counterstained with Hoechst 33342 (blue). Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.
Image courtesy of Michael W. Davidson, National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL.
Image courtesy of Michael W. Davidson, National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL.
BioSpotlight
BioTechniques, Vol. 42, No. 4, April 2007, pp. 403–405
doi
10.2144/000112438
Citations
Nijsje Dorman
BioTechniques, Vol. 42, No. 4, April 2007, pp. 407–409
doi
10.2144/000112439
Scientists
Christie Aschwanden
BioTechniques, Vol. 42, No. 4, April 2007, p. 411
doi
10.2144/000112440
About the Authors
BioTechniques, Vol. 42, No. 4, April 2007, p. 413
doi
10.2144/000112445
BioTechniques, Vol. 42, No. 4, April 2007, p. 415
doi
10.2144/000112446
WebWatch
Kevin Ahern
BioTechniques, Vol. 42, No. 4, April 2007, p. 417
doi
10.2144/000112442
Tech News
Lynne Lederman
BioTechniques, Vol. 42, No. 4, April 2007, pp. 419–423
doi
10.2144/000112443
Tech Insight
Marc D. Roy
BioTechniques, Vol. 42, No. 4, April 2007, pp. 425–435
doi
10.2144/000112441
Short Technical Reports
Amanda B. Hummon,
Sharlene R. Lim,
Michael J. Difilippantonio,
and Thomas Ried
BioTechniques, Vol. 42, No. 4, April 2007, pp. 467–472
doi
10.2144/000112401
Han Yen Tan,
Tuck Wah Ng,
and Oi Wah Liew
BioTechniques, Vol. 42, No. 4, April 2007, pp. 474–478
doi
10.2144/000112402
Helena Korpelainen,
Kirsi Kostamo,
and Viivi Virtanen
BioTechniques, Vol. 42, No. 4, April 2007, pp. 479–486
doi
10.2144/000112415
Indranil Biswas,
Laura Drake,
Sean Johnson,
and Damon Thielen
BioTechniques, Vol. 42, No. 4, April 2007, pp. 487–490
doi
10.2144/000112414
Research Reports
Sung O. Kim,
Soon-Duck Ha,
Sangun Lee,
Sarah Stanton,
Bruce Beutler,
and Jiahuai Han
BioTechniques, Vol. 42, No. 4, April 2007, pp. 493–501
doi
10.2144/000112390
Jenny Hong Cai,
Shibing Deng,
Steven W. Kumpf,
Patricia A. Lee,
Panayiotis Zagouras,
Anne Ryan,
and Dan S. Gallagher
BioTechniques, Vol. 42, No. 4, April 2007, pp. 503–512
doi
10.2144/000112400
Benchmarks
Jhanvi H. Dangaria,
Sung Yang,
and Peter J. Butler
BioTechniques, Vol. 42, No. 4, April 2007, pp. 437–440
doi
10.2144/000112427
Victor Vassar,
Crystal Hagen,
Jeffrey Ludwig,
Richard Thomas,
and Jiming Zhou
BioTechniques, Vol. 42, No. 4, April 2007, pp. 442–444
doi
10.2144/000112435
Lijuan Zhang,
Nadya G. Gurskaya,
Ekaterina M. Merzlyak,
Dmitry B. Staroverov,
Nikolay N. Mudrik,
Olga N. Samarkina,
Leonid M. Vinokurov,
Sergey Lukyanov,
and Konstantin A. Lukyanov
BioTechniques, Vol. 42, No. 4, April 2007, pp. 446–450
doi
10.2144/000112453
Barbara Bartolini,
Anna Rosa Garbuglia,
Douglas Horejsh,
Federico Martini,
Fabrizio Carletti,
and Maria Rosaria Capobianchi
BioTechniques, Vol. 42, No. 4, April 2007, pp. 452–456
doi
10.2144/000112450
Andrew A. Forbes,
Thomas H.Q. Powell,
Neil F. Lobo,
and Jeffrey L. Feder
BioTechniques, Vol. 42, No. 4, April 2007, pp. 458–462
doi
10.2144/000112452
Application Forum
Jean Qiu, PhD,
and Peter Y. Li, PhD
BioTechniques, Vol. 42, No. 4, April 2007, p. 513
doi
10.2144/000112471
