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February 2007 Articles
Volume:42 , Issue Number:2
Cover Image:
Mouse intestine stained for laminin (Cy™3), filamentous actin (phalloidin conjugated to Alexa Fluor® 488), and nuclear DNA (DRAQ5™). Images were recorded in grayscale with a 12- bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.
Image courtesy of Michael W. Davidson, National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL.
BioSpotlight
BioSpotlight
BioTechniques, Vol. 42, No. 2, February 2007, pp. 115–117
doi  10.2144/000112392
 
Citations
Citations
Nathan Blow, and Nijsje Dorman
BioTechniques, Vol. 42, No. 2, February 2007, pp. 119–121
doi  10.2144/000112393
 
Scientists
Profile of Bonnie L. Bassler, Ph.D.
Lynne Lederman
BioTechniques, Vol. 42, No. 2, February 2007, p. 123
doi  10.2144/000112394
Profile of C. David Allis, Ph.D.
Christie Aschwanden
BioTechniques, Vol. 42, No. 1, January 2007, p. 19
doi  10.2144/000112338
 
About the Authors
The Rao Laboratory
BioTechniques, Vol. 42, No. 2, February 2007, p. 125
doi  10.2144/000112399
The Molecular Genetics and Sequencing Research Group
BioTechniques, Vol. 42, No. 2, February 2007, p. 127
doi  10.2144/000112398
 
WebWatch
WebWatch
Kevin Ahern
BioTechniques, Vol. 42, No. 2, February 2007, p. 129
doi  10.2144/000112395
 
Tech News
Chemical Biology
Lynne Lederman
BioTechniques, Vol. 42, No. 2, February 2007, pp. 131–135
doi  10.2144/000112397
 
Tech Insight
Application of protein-fragment complementation assays in cell biology
Ingrid Remy, and Stephen W. Michnick
BioTechniques, Vol. 42, No. 2, February 2007, pp. 137–145
doi  10.2144/000112396
 
BioFeedback
Optimization of fluorescence measurement in duplex real-time PCR with TaqMan® probes labeled with VIC and quenched by TAMRA
Tomáš Kuchta, Ph.D., Klára Krascsenicsová, and Gabriel Bánréti
BioTechniques, Vol. 42, No. 2, February 2007, pp. 147–149
doi  10.2144/000112391
 
Short Technical Reports
High correspondence between Affymetrix exon and standard expression arrays
Michał J. Okoniewski, Yvonne Hey, Stuart D. Pepper, and Crispin J. Miller
BioTechniques, Vol. 42, No. 2, February 2007, pp. 181–185
doi  10.2144/000112315
Regeneration of commercial nucleic acid extraction columns without the risk of carryover contamination
Nagadenahalli B. Siddappa, Appukuttan Avinash, Mohanram Venkatramanan, and Udaykumar Ranga
BioTechniques, Vol. 42, No. 2, February 2007, pp. 186–192
doi  10.2144/000112327
Multiplex genotyping by melting analysis of loci-spanning probes: β-globin as an example
Genevieve Pont-Kingdon, Lan-Szu Chou, Kristy Damjanovich, Kelli Sumner, Mark Herrmann, Maria Erali, and Elaine Lyon
BioTechniques, Vol. 42, No. 2, February 2007, pp. 193–197
doi  10.2144/000112330
 
Research Reports
Universal reference method for real-time PCR gene expression analysis of preimplantation embryos
Neil Ivan Bower, Ralf Joachim Moser, Jonathan Robert Hill, and Sigrid Arabella Lehnert
BioTechniques, Vol. 42, No. 2, February 2007, pp. 199–206
doi  10.2144/000112314
Magnetic resonance-based visualization of gene expression in mammalian cells using a bacterial polyphosphate kinase reporter gene
Sewon Ki, Fuminori Sugihara, Koji Kasahara, Hidehito Tochio, Masahiro Shirakawa, and Tetsuro Kokubo
BioTechniques, Vol. 42, No. 2, February 2007, pp. 209–215
doi  10.2144/000112319
Integration site-specific transcriptional reporter gene analysis using Flp recombinase targeted cell lines
Mahdad Karimi, Lauren C. Goldie, Daniela Ulgiati, and Lawrence J. Abraham
BioTechniques, Vol. 42, No. 2, February 2007, pp. 217–224
doi  10.2144/000112317
 
Benchmarks
Amino terminal fusion of heterologous proteins to CotC increases display efficiencies in the Bacillus subtilis spore system
Rachele Isticato, Donatella Scotto Di Mase, Emilia M.F. Mauriello, Maurilio De Felice, and Ezio Ricca
BioTechniques, Vol. 42, No. 2, February 2007, pp. 151–156
doi  10.2144/000112329
Proteasome inhibition in wild-type yeast Saccharomyces cerevisiae cells
Chang Liu, Jennifer Apodaca, Laura E. Davis, and Hai Rao
BioTechniques, Vol. 42, No. 2, February 2007, pp. 158–162
doi  10.2144/000112389
CaSpeR5, a family of Drosophila transgenesis and shuttle vectors with improved multiple cloning sites
Tien Le, Marcus Yu, Brandon Williams, Sagar Goel, Sarah M. Paul, and Greg J. Beitel
BioTechniques, Vol. 42, No. 2, February 2007, pp. 164–166
doi  10.2144/000112386
Minimization of between-well sample variance of antifungal activity using a high-throughput screening microplate bioassay
Scott W. Pryor, Donna M. Gibson, Gary C. Bergstrom, and Larry P. Walker
BioTechniques, Vol. 42, No. 2, February 2007, pp. 168–172
doi  10.2144/000112328
5′-Tailed sequencing primers improve sequencing quality of PCR products
Jonas Binladen, M. Thomas, P. Gilbert, Paula F. Campos, and Eske Willerslev
BioTechniques, Vol. 42, No. 2, February 2007, pp. 174–176
doi  10.2144/000112316
 
Application Forum - Sponsored Paper
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
Chad Brueck, Sunny Song, and Jim Collins
BioTechniques, Vol. 42, No. 2, February 2007, pp. 230–233
doi  10.2144/000112417
 

 
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