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In the article by B. Afrough et al. (BioTechniques 43:458-462, doi 10.2144/000112554), the graph legend in (Figure 2)C was inadvertently excluded. The complete figure and its legend appear below.
Figure 2.
Monosaccharide pre-inhibition of positive lectin binders to mucin glycoprotein as identified by the Synblock method. Lectin inhibition experiments using conditions from the Synblock-optimized model, the lectins (A) Ulex europaeus agglutinin I (UEA-I), (B) Jacalin, (C) soybean agglutinin (SBA), (D) wheat germ agglutinin (WGA), and (E) Ricinus communis agglutinin I (RCA-I) were pre-incubated with a range of concentrations of different monosaccharides coded in the legend by (Δ) L-fucose, (•) D-galactose, (◊) D-fucose, (○) D-mannose, (▪) N-acetyl D-glucosamine (GlcNAc), and (□) N-acetyl D-galactosamine (GalcNAc) as shown. The results are mean absorbance values from three independent experiments, and error bars represent the standard deviation from the mean. The initial value (no inhibiting sugar) is the mean value of six experiments undertaken in the absence of inhibiting sugar. The negative control was carried out in the absence of glycoprotein represented as Mucin-ve.
The BioTechniques staff sincerely regrets this oversight. The PDF and HTML versions of the article, available at www.BioTechniques.com , have been corrected.
