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October 2007 Articles
Volume:43 , Issue Number:4
Cover Image:
Laser scanning confocal image of Neuro-2a mouse neuroblastoma cells. Microtubules (green) were stained with an antibody to -tubulin and an AlexaFluor® 488 conjugated secondary antibody. Actin filaments (red) were labeled with Texas Red® phalloidin and cell nuclei (blue) were stained with Hoechst 33342.
Image courtesy of Gordon Ruthel, Cell Imaging Center, U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), Frederick, MD.
Introduction
Who are you?
Douglas McCormick
BioTechniques, Vol. 43, No. 4, October 2007, p. Si
doi  10.2144/000112586
 
From the Editor
A Positive Need for Negative Data
Douglas McCormick
BioTechniques, Vol. 43, No. 4, October 2007, p. 389
doi  10.2144/000112576
 
BioSpotlight
BioSpotlight
BioTechniques, Vol. 43, No. 4, October 2007, pp. 391–393
doi  10.2144/000112569
 
Citations
Citations
Nijsje Dorman
BioTechniques, Vol. 43, No. 4, October 2007, pp. 395–397
doi  10.2144/000112570
 
Scientists
Profile of Elizabeth H. Blackburn, Ph.D.
Lynne Lederman
BioTechniques, Vol. 43, No. 4, October 2007, p. 399
doi  10.2144/000112567
 
About the Authors
The Smart Amplification Process Team
BioTechniques, Vol. 43, No. 4, October 2007, p. 401
doi  10.2144/000112573
Laboratory of Karl Klose
BioTechniques, Vol. 43, No. 4, October 2007, p. 403
doi  10.2144/000112565
 
WebWatch
WebWatch
Kevin Ahern
BioTechniques, Vol. 43, No. 4, October 2007, p. 405
doi  10.2144/000112572
 
Tech News
Sequencing
Lynne Lederman
BioTechniques, Vol. 43, No. 4, October 2007, pp. 407–411
doi  10.2144/000112571
 
Tech Insight
Electron Cryotomography
Gavin E. Murphy, and Grant J. Jensen
BioTechniques, Vol. 43, No. 4, October 2007, pp. 413–421
doi  10.2144/000112568
 
NIH Grant Watch
NIH Grant Watch
BioTechniques, Vol. 43, No. 4, October 2007, p. 521
doi  10.2144/000112577
 
BioFeedback
BioFeedback
BioTechniques, Vol. 43, No. 4, October 2007, p. 425
doi  10.2144/000112587
 
Review
Phase separation in the isolation and purification of membrane proteins
Thomas Arnold, and Dirk Linke
BioTechniques, Vol. 43, No. 4, October 2007, pp. 427–440
doi  10.2144/000112566
Forensic DNA fingerprinting by liquid chromatography-electrospray ionization mass spectrometry
Herbert Oberacher, and Walther Parson
BioTechniques, Vol. 43, No. 4, October 2007, pp. Svii–Sxiii
doi  10.2144/000112581
 
Short Technical Reports
Use of a competitive probe in assay design for genotyping of the UGT1A1*28 microsatellite polymorphism by the smart amplification process
Jun Watanabe, Yasumasa Mitani, Yuki Kawai, Takeshi Kikuchi, Yasushi Kogo, Atsuko Oguchi-Katayama, Hajime Kanamori, Kengo Usui, Masayoshi Itoh, Paul E. Cizdziel, Alexander Lezhava, Kenji Tatsumi, Yasushi Ichikawa, Shinji Togo, Hiroshi Shimada, and Yoshihide Hayashizaki
BioTechniques, Vol. 43, No. 4, October 2007, pp. 479–484
doi  10.2144/000112563
Construction of targeted insertion mutations in Francisella tularensis subsp. novicida
Jirong Liu, Xhavit Zogaj, Jeffrey R. Barker, and Karl E. Klose
BioTechniques, Vol. 43, No. 4, October 2007, pp. 487–492
doi  10.2144/000112574
Scaffold-free three-dimensional cell culture utilizing micromolded nonadhesive hydrogels
Anthony P. Napolitano, Dylan M. Dean, Alan J. Man, Jacquelyn Youssef, Don N. Ho, Adam P. Rago, Matthew P. Lech, and Jeffrey R. Morgan
BioTechniques, Vol. 43, No. 4, October 2007, pp. 494–500
doi  10.2144/000112591
Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids
Robert H. Batchelor, Adam Sarkez, W. Gregory Cox, and Iain Johnson
BioTechniques, Vol. 43, No. 4, October 2007, pp. 503–507
doi  10.2144/000112564
 
Research Reports
Improved agarose gel assay for quantification of growth factor-induced cell motility
Yoanne Mousseau, Dany Leclers, Karine Faucher-Durand, Jeanne Cook-Moreau, Anne-Sophie Lia-Baldini, Michel Rigaud, and Franck G. Sturtz
BioTechniques, Vol. 43, No. 4, October 2007, pp. 509–516
doi  10.2144/000112557
 
Benchmarks
Self-assembly of proximity probes for flexible and modular proximity ligation assays
Spyros Darmanis, Anna Kähler, Lena Spångberg, Masood Kamali-Moghaddam, Ulf Landegren, and Edith Schallmeiner
BioTechniques, Vol. 43, No. 4, October 2007, pp. 443–450
doi  10.2144/000112551
TraceHaplotyper: using direct sequencing to determine the phase of an indel followed by biallelic SNPs
Yanir Seroussi, and Eyal Seroussi
BioTechniques, Vol. 43, No. 4, October 2007, pp. 452–456
doi  10.2144/000112552
Identification and elimination of false-positives in an ELISA-based system for qualitative assessment of glycoconjugate binding using a selection of plant lectins
Babak Afrough, Miriam V. Dwek, and Pamela Greenwell
BioTechniques, Vol. 43, No. 4, October 2007, pp. 458–464
doi  10.2144/000112554
Improved recombinational stability of lentiviral expression vectors using reduced-genome Escherichia coli
Chacko S. Chakiath, and Dominic Esposito
BioTechniques, Vol. 43, No. 4, October 2007, pp. 466–470
doi  10.2144/000112585
Epifluorescent microscopy to evaluate bacteriophage capsid integrity
Kerusha Naicker, and Steven I. Durbach
BioTechniques, Vol. 43, No. 4, October 2007, pp. 473–476
doi  10.2144/000112584
 
Application Forum
Simultaneous Quantification of Four RNA Targets by Multiplex, Real-Time RT-PCR without Optimization
John W. Coleman, J. Erik Johnson, and David K. Clarke
BioTechniques, Vol. 43, No. 4, October 2007, pp. 517–519
doi  10.2144/000112626
 
Mini-Reviews
Short tandem repeat typing technologies used in human identity testing
John M. Butler
BioTechniques, Vol. 43, No. 4, October 2007, pp. Sii–Sv
doi  10.2144/000112582
 


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