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June 2009
Volume:46 , Issue Number:7
Cover Image:
High-resolution confocal image of a brain blood vessel in a fixed brain tissue section from a monkey infected with adenovirus. The section is fluorescently labeled for T cells (green), memory T cells (red), and counterstained to highlight the cell nuclei (blue). Imaged using a Leica DMIRB/IRE2 confocal imaging system. PFA fixed brain vibratome sections (green: Alexa 488, labeling CD3 for T cells; red: Alexa 594, labeling CD45RO for memory T cells; blue: DAPI as a counterstaining). Courtesy of Carlos Barcia, Clinical and Experimental Neuroscience, School of Medicine, University of Murcia, Spain and Gene Therapeutics Research Institute, University of California, Los Angeles/Cedars-Sinai Medical Center, Los Angeles, CA USA.
From the Editor
Can We Really Hope to Control Healthcare Spending?
Douglas McCormick
BioTechniques, Vol. 46, No. 7, June 2009, p. 493
 
BioSpotlight
BioSpotlight
Patrick C.H. Lo, Ph.D., Kristie Nybo, Ph.D.
BioTechniques, Vol. 46, No. 7, June 2009, p. 494
 
Citations
Citations
Nijsje Dorman, Ph.D.
BioTechniques, Vol. 46, No. 7, June 2009, p. 495
 
Education Outreach
Discover the Microbes Within! The HHMI Wolbachia Project
Michael P. Weiner, and Barton Slatko
BioTechniques, Vol. 46, No. 7, June 2009, p. 497
 
WebWatch
WebWatch
Kevin Ahern, Ph.D.
BioTechniques, Vol. 46, No. 7, June 2009, p. 499
 
Tech News
Bioinformatics and Systems Biology
Lynne Lederman, Ph.D.
BioTechniques, Vol. 46, No. 7, June 2009, pp. 501–503
 
Troubleshooting Forum
DNA and General PCR Methods: PCR Primer Design
Kristie Nybo, Ph.D.
BioTechniques, Vol. 46, No. 7, June 2009, pp. 505–507
 
NIH Grant Watch
The NIH and Economic Recovery
Kristie Nybo, Ph.D.
BioTechniques, Vol. 46, No. 7, June 2009, p. 509
 
Short Technical Reports
Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR
Clare E. Holleley, Paul G. Geerts
BioTechniques, Vol. 46, No. 7, June 2009, pp. 511–517
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Prithiviraj Jothikumar, Vincent Hill, Jothikumar Narayanan
BioTechniques, Vol. 46, No. 7, June 2009, pp. 519–524
Site-directed mutagenesis of multi-copy-number plasmids: Red/ET recombination and unique restriction site elimination
Stephan Noll, Gabriele Hampp, Hanna Bausbacher, Natalia S. Pellegata, Harald Kranz
BioTechniques, Vol. 46, No. 7, June 2009, pp. 527–533
 
Research Reports
Transposon-directed base-exchange mutagenesis (TDEM): a novel method for multiple-nucleotide substitutions within a target gene
Yun Cheol Kim, Hui Sun Lee, Sukjoon Yoon, Sherie L. Morrison
BioTechniques, Vol. 46, No. 7, June 2009, pp. 534–542
Simple non-fluorescent polarity labeling of microtubules for molecular motor assays
Virupakshi Soppina, Arpan K. Rai, Roop Mallik
BioTechniques, Vol. 46, No. 7, June 2009, pp. 543–549
 
Benchmarks
Mouse embryo cryopreservation utilizing a novel high-capacity vitrification spatula
Wai Hung Tsang, King L. Chow
BioTechniques, Vol. 46, No. 7, June 2009, pp. 550–552
A device for automated hydrodynamic shearing of genomic DNA
Aric Joneja, Xiaohua Huang
BioTechniques, Vol. 46, No. 7, June 2009, pp. 553–556
 
Application Forum – Sponsored Paper
Gene Expression Analysis of Paclitaxel-Treated HT29 Cells Using the xCELLigence™ System and RealTime ready™ Panels
Tanja Scheuermann, Yuli Sun, Susanne Sagner, Barbara Rueger
BioTechniques, Vol. 46, No. 7, June 2009, pp. 557–558
Simple, Fast, and Efficient Cloning of PCR Products with TOPO® Cloning Vectors
Balwant Patel
BioTechniques, Vol. 46, No. 7, June 2009, p. 559
 


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