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March 2009
Volume:46 , Issue Number:3
Cover Image:
Cultured human mesenchymal stem cells stained live for mitochondria (MitoTracker Deep Red, purple), and subsequently fixed with 4% formaldehyde and stained for nuclei (DAPI, blue), integrin β2 subunit (anti-CD29, green), and F-actin filament (phalloidin Alexa 546, yellow). Images were captured on a Nikon Eclipse TE300 inverted fluorescent microscope with 20× objective, monochromatic Spot Insight2 digital camera, and Semrock panel of Brightline fluor filter cubes. Four separate images with preassigned colors were merged into a single full-color image using Spot Advanced imaging software. Image courtesy of Shayne Boucher, Life Technologies, GIBCO Cell Culture, Primary & Stem Cell Systems, Grand Island, NY, USA.
From the Editor
Douglas McCormick
BioTechniques, Vol. 46, No. 3, March 2009, p. 149
doi  10.2144/000113096
 
BioSpotlight
Patrick C.H. Lo, Ph.D., and Kristie Nybo, Ph.D.
BioTechniques, Vol. 46, No. 3, March 2009, p. 151
doi  10.2144/000113097
 
Citations
Nijsje Dorman, Ph.D.
BioTechniques, Vol. 46, No. 3, March 2009, p. 153
doi  10.2144/000113098
 
Scientists
Kristie Nybo, Ph.D.
BioTechniques, Vol. 46, No. 3, March 2009, p. 155
doi  10.2144/000113129
 
WebWatch
Kevin Ahern, Ph.D.
BioTechniques, Vol. 46, No. 3, March 2009, p. 157
doi  10.2144/000113101
 
Tech News
Lynne Lederman, Ph.D.
BioTechniques, Vol. 46, No. 3, March 2009, pp. 159–161
doi  10.2144/000113102
 
Troubleshooting Forum
Kristie Nybo, Ph.D.
BioTechniques, Vol. 46, No. 3, March 2009, pp. 163–165
doi  10.2144/000113147
 
BioFeedback
BioTechniques, Vol. 46, No. 3, March 2009, p. 142
doi  10.2144/000113106
 
Short Technical Reports
Motoki Goto, Eiichi Honda, Atsuo Ogura, Akio Nomoto, and Ken-Ichi Hanaki, D.V.M.
BioTechniques, Vol. 46, No. 3, March 2009, pp. 167–172
doi  10.2144/000113072
Xiaofang Jin, Jessica Rose Newton, Stephen Montgomery-Smith, and George P. Smith
BioTechniques, Vol. 46, No. 3, March 2009, pp. 175–182
doi  10.2144/000113074
Jawad Abdelkrim, Bruce C. Robertson, Jo-Ann L. Stanton, and Neil J. Gemmell
BioTechniques, Vol. 46, No. 3, March 2009, pp. 185–192
doi  10.2144/000113084
Morten E. Allentoft, Stephan C. Schuster, Richard N. Holdaway, Marie L. Hale, Emma McLay, Charlotte Oskam, M. Thomas P. Gilbert, Peter Spencer, Eske Willerslev, and Michael Bunce
BioTechniques, Vol. 46, No. 3, March 2009, pp. 195–200
doi  10.2144/000113086
 
Research Reports
Jian-Yong Xu, Gen-Bo Xu, and Song-Lin Chen
BioTechniques, Vol. 46, No. 3, March 2009, pp. 201–208
doi  10.2144/000113075
Jane P. Bearinger, Lawrence C. Dugan, Ligang Wu, Haley Hill, Allen T. Christian, and Jeffrey A. Hubbell
BioTechniques, Vol. 46, No. 3, March 2009, pp. 209–216
doi  10.2144/000113073
Quentin C. Santana, Martin P. A. Coetzee, Emma T. Steenkamp, Osmond X. Mlonyeni, Gifty N. A. Hammond, Michael J. Wingfield, and Brenda D. Wingfield
BioTechniques, Vol. 46, No. 3, March 2009, pp. 217–223
doi  10.2144/000113085
 
Benchmarks
Shigeru Matsunaga, Qiuhong Xie, Miyuki Kumano-Kuramochi, Shiro Komba, and Sachiko Machida
BioTechniques, Vol. 46, No. 3, March 2009, pp. 225–227
doi  10.2144/000113071
Olivier Harismendy, and Kelly A. Frazer
BioTechniques, Vol. 46, No. 3, March 2009, pp. 229–231
doi  10.2144/000113082
 
Application Forum - Sponsored Paper
Applied Biosystems Inc.
BioTechniques, Vol. 46, No. 3, March 2009, pp. 232–234
doi  10.2144/000113128