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Mini-PROTEAN® TGX™ precast gel for SDS-PAGE with improved stability: comparison with standard Laemmli gels
 

BioTechniques, Vol. 48, No. 2, February 2010

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Abstract

Bio-Rad’s Mini-PROTEAN TGX gels for SDS-PAGE are based on a modification of the Laemmli system that gives significantly improved stability and performance over time. Mini-PROTEAN TGX gels retain separation characteristics similar to Laemmli gels and use standard running and sample buffers. In this study, the performance of TGX gels and standard Laemmli system precast gels are compared following storage at 37ÂșC to mimic accelerated assay conditions, and the separation performance and accuracy of molecular weight estimation of both gel systems are presented.




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