to BioTechniques free email alert service to receive content updates.
November 2012
Volume:53 , Issue Number:5
Cover Image:
Ratiometric comparison of three fluorophores expressed from the same T-DNA. Exemplary CLSM image of epidermal cells from leaves of N. tabacum two days after infiltration with Agrobacterium tumefaciens containing pBiFCt-2in1-NN (ECFP/EGFP, above) and pBiFCt-2in1-NN (EGFP/ECFP, below). Scale bar, 30 M. Images were taken using an LSM510 microscope from Zeiss (Cambridge, UK) with standardized excitation intensities and photomultiplier gains and with the following excitation wavelength/emission bandwidth/dichroic filter settings: 458 nm/LP475/HFT458/543 (secondary dichroic 490) (CFP), 488 nm/BP505–530/HFT488/543 (GFP), and 543 nm/BP560–615/HFT488/543 (RFP). Bleed-through from CFP into the GFP channel was checked using separate controls with the same settings. Image from C. Grefen and M.R. Blatt (pg 311). Cover design by Genevieve McCarthy
From the Editor
Nathan S. Blow, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 267
doi  10.2144/000113944
 
BioSpotlight
Patrick Lo, Ph.D., and Kristie Nybo, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 269
doi  10.2144/000113945
 
Citations
Nijsje Dorman, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 271
doi  10.2144/000113946
 
Scientists
Kristie Nybo, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 273
doi  10.2144/000113947
 
Tech News
Jeffrey M. Perkel, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, pp. 275–281
doi  10.2144/000113949
 
Troubleshooting Forum
Kristie Nybo, Ph. D.
BioTechniques, Vol. 53, No. 5, November 2012, pp. 282–283
doi  10.2144/000113950
 
Review
Yutaka Kodama, and Chang-Deng Hu
BioTechniques, Vol. 53, No. 5, November 2012, pp. 285–298
doi  10.2144/000113943
 
Benchmarks
Perfecto Santiago-Sotelo, and Jorge Humberto Ramirez-Prado
BioTechniques, Vol. 53, No. 5, November 2012, pp. 299–300
doi  10.2144/000113953
Ari Lehmusvuori, Julius Manninen, Tuomas Huovinen, Tero Soukka, and Urpo Lamminmäki
BioTechniques, Vol. 53, No. 5, November 2012, pp. 301–303
doi  10.2144/0000113954
 
Rapid Dispatches
Lilia Y. Kucheryavykh, Yuriy V. Kucheryavykh, Kimberleve Rolón-Reyes, Serguei N. Skatchkov, Misty J. Eaton, Luis A. Cubano, and Mikhail Inyushin
BioTechniques, Vol. 53, No. 5, November 2012, pp. 305–309
doi  10.2144/000113940
Christopher Grefen, and Michael R. Blatt
BioTechniques, Vol. 53, No. 5, November 2012, pp. 311–314
doi  10.2144/000113941
Anil B. Shrirao, Ali Hussain, Cheul H. Cho, and Raquel Perez-Castillejos
BioTechniques, Vol. 53, No. 5, November 2012, pp. 315–318
doi  10.2144/000113928