November 2012
Volume:53 , Issue Number:5
Cover Image:
Ratiometric comparison of three fluorophores expressed from the same T-DNA. Exemplary CLSM image of epidermal cells from leaves of N. tabacum two days after infiltration with Agrobacterium tumefaciens containing pBiFCt-2in1-NN (ECFP/EGFP, above) and pBiFCt-2in1-NN (EGFP/ECFP, below). Scale bar, 30 M. Images were taken using an LSM510 microscope from Zeiss (Cambridge, UK) with standardized excitation intensities and photomultiplier gains and with the following excitation wavelength/emission bandwidth/dichroic filter settings: 458 nm/LP475/HFT458/543 (secondary dichroic 490) (CFP), 488 nm/BP505–530/HFT488/543 (GFP), and 543 nm/BP560–615/HFT488/543 (RFP). Bleed-through from CFP into the GFP channel was checked using separate controls with the same settings. Image from C. Grefen and M.R. Blatt (pg 311). Cover design by Genevieve McCarthy
From the Editor
Needle in a haystack
Nathan S. Blow, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 267
doi  10.2144/000113944
 
BioSpotlight
BioSpotlight
Patrick Lo, Ph.D., and Kristie Nybo, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 269
doi  10.2144/000113945
 
Citations
Citations
Nijsje Dorman, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 271
doi  10.2144/000113946
 
Scientists
Profile of Robert Langer
Kristie Nybo, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, p. 273
doi  10.2144/000113947
 
Tech News
Fresh views on DNA structure
Jeffrey M. Perkel, Ph.D.
BioTechniques, Vol. 53, No. 5, November 2012, pp. 275–281
doi  10.2144/000113949
 
Troubleshooting Forum
Immunohistochemistry: background staining
Kristie Nybo, Ph. D.
BioTechniques, Vol. 53, No. 5, November 2012, pp. 282–283
doi  10.2144/000113950
 
Review
Bimolecular fluorescence complementation (BiFC): A 5-year update and future perspectives
Yutaka Kodama, and Chang-Deng Hu
BioTechniques, Vol. 53, No. 5, November 2012, pp. 285–298
doi  10.2144/000113943
 
Benchmarks
prfectBLAST: a platform-independent portable front end for the command terminal BLAST+ stand-alone suite
Perfecto Santiago-Sotelo, and Jorge Humberto Ramirez-Prado
BioTechniques, Vol. 53, No. 5, November 2012, pp. 299–300
doi  10.2144/000113953
Homogenous M13 bacteriophage quantification assay using switchable lanthanide fluorescence probes
Ari Lehmusvuori, Julius Manninen, Tuomas Huovinen, Tero Soukka, and Urpo Lamminmäki
BioTechniques, Vol. 53, No. 5, November 2012, pp. 301–303
doi  10.2144/0000113954
 
Rapid Dispatches
Visualization of implanted GL261 glioma cells in living mouse brain slices using fluorescent 4-(4-(dimethylamino)-styryl)-N-methylpyridinium iodide (ASP+)
Lilia Y. Kucheryavykh, Yuriy V. Kucheryavykh, Kimberleve Rolón-Reyes, Serguei N. Skatchkov, Misty J. Eaton, Luis A. Cubano, and Mikhail Inyushin
BioTechniques, Vol. 53, No. 5, November 2012, pp. 305–309
doi  10.2144/000113940
A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC)
Christopher Grefen, and Michael R. Blatt
BioTechniques, Vol. 53, No. 5, November 2012, pp. 311–314
doi  10.2144/000113941
Adhesive-tape soft lithography for patterning mammalian cells: application to wound-healing assays
Anil B. Shrirao, Ali Hussain, Cheul H. Cho, and Raquel Perez-Castillejos
BioTechniques, Vol. 53, No. 5, November 2012, pp. 315–318
doi  10.2144/000113928
 

 
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