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Peptide-conjugated glass slides for selective capture and purification of diagnostic cells: Applications in urine cytology
 
Danuta B. Wronska, Magdalena Krajewska, Natalia Lygina, Juhua C. Morrison, Dalia Juzumiene, W. David Culp, Shrikumar A. Nair, Martyn Darby, and Christopher M. Hofmann
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Table 5. 









The work presented here demonstrates a novel technique for epithelial cell capture from urine samples for slide-based analysis. In particular, we demonstrated a slide configuration that can capture and isolate scarce urothelial cells found in human urine, even in the presence of contaminating blood cells. Importantly, the slides are compatible with Papanicolaou staining for urine cytology and can be used with ICC for detecting cancer marker expression. Thus, our peptide-conjugated slide technology represents a powerful new technique for preparing a purified cell population for diagnostic testing.

Author contributions

D.B.W., W.D.C., N.L., M.K., D.J., and J.C.M. conceived and developed the experiments presented here and analyzed the resulting data. M.K., M.D., and S.A.N. established protocols for peptide conjugation to glass. M.K. conjugated the peptides to the slides and performed peptide density quantification. N.L. and D.J. per formed cell capture assays. J.C.M. and N.L. performed cytology staining and ICC experiments. W.D.C. performed phage display and isolated the epithelial cell binding peptides. S.A.N. was responsible for peptide design and manufacturing. D.B.W. provided leadership to the project. C.M.H. wrote and revised the manuscript, and all authors read and approved the text.

Acknowledgments

We acknowledge William Siesser for his role in developing the initial ideas for peptide-conjugated glass, Jonathan White, Yuchen Chen, and Phil Hamilton for synthesis and purification of the cell binding peptides, Wesley Storm for his help editing the manuscript, and Bruce Lamb for his guidance as head of the R&D group at Affinergy.

Competing interests

All authors were employees of Affinergy at the time this work was completed. The peptides and methods described here are contained in an unpublished patent application filed by Affinergy. There are no marketed products to declare.

Correspondence
Address correspondence to Martyn Darby, Affinergy, LLC, Research Triangle Park, NC. E-mail: [email protected]">[email protected]

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