http://www.biotechniques.com/BiotechniquesJournal/ In the Journal en-us ℗ & © Wed Jan 16 19:44:04 GMT 2013 of Informa Pharma. Please refer to the Terms and Conditions of Use at http://www.biotechniques.com rss@biotechniques.com Fri, 11 Jan 2013 19:43:03 +0000 Wed, 16 Jan 2013 19:44:04 +0000 60 In the Journal http://www.biotechniques.com/BiotechniquesJournal/2013/January/BioSpotlight/biotechniques-339024.html Fri, 11 Jan 2013 19:43:03 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/BioSpotlight/biotechniques-339024.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/Profile-of-David-Reich/biotechniques-339022.html Fri, 11 Jan 2013 19:34:03 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/Profile-of-David-Reich/biotechniques-339022.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/Homopolymer-tail-mediated-ligation-PCR-a-streamlined-and-highly-efficient----method-for-DNA-cloning-and-library-construction/biotechniques-339020.html The amplification of DNA fragments, cloned between user-defined 5′ and 3′ end sequences, is a prerequisite step in the use of many current applications including massively parallel sequencing (MPS). Here we describe an improved ... Mon, 14 Jan 2013 16:24:15 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/Homopolymer-tail-mediated-ligation-PCR-a-streamlined-and-highly-efficient----method-for-DNA-cloning-and-library-construction/biotechniques-339020.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/A-novel-method-for-genetic-transformation-of-yeast-cells-using-oligoelectrolyte-polymeric-nanoscale-carriers/biotechniques-339017.html The genetic transformation of target cells is a key tool in modern biological research, as well as in many gene therapy and biotechnology applications. Here we describe a new method for delivery of DNA into several industrially important species of... Fri, 11 Jan 2013 19:19:14 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/A-novel-method-for-genetic-transformation-of-yeast-cells-using-oligoelectrolyte-polymeric-nanoscale-carriers/biotechniques-339017.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/Co-transfection-of-Plasmid-DNA/biotechniques-339008.html Fri, 11 Jan 2013 19:13:04 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/Co-transfection-of-Plasmid-DNA/biotechniques-339008.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/qPCR-Single-copy-targets/biotechniques-339005.html Fri, 11 Jan 2013 19:04:01 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/qPCR-Single-copy-targets/biotechniques-339005.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/Rewriting-history-through-proteins/biotechniques-339003.html Today, researchers interested in exploring ancient samples and remains at the molecular level mainly rely on DNA. But proteins might tell us even more about history. Andrew Wiecek examines how protein analysis techniques are shedding new light on Inca... Fri, 11 Jan 2013 18:59:05 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/Rewriting-history-through-proteins/biotechniques-339003.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/Citations/biotechniques-339001.html Fri, 11 Jan 2013 18:52:03 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/Citations/biotechniques-339001.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/30-years-of-methods/biotechniques-338999.html Fri, 11 Jan 2013 18:47:01 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/30-years-of-methods/biotechniques-338999.html http://www.biotechniques.com/BiotechniquesJournal/2011/December/Profile-of-Tracy-L.-Bale/biotechniques-338811.html Mon, 07 Jan 2013 18:41:32 +0000 http://www.biotechniques.com/BiotechniquesJournal/2011/December/Profile-of-Tracy-L.-Bale/biotechniques-338811.html http://www.biotechniques.com/BiotechniquesJournal/2013/January/Separation-of-integument-and-nucellar-tissues-from-cotton-ovules-Gossypium-hirsutum-L.-for-both-high--and-low-throughput-molecular-applications/biotechniques-338247.html Here we present a quick and low-cost method to separate the different layers of tissue from the ovules and young seeds of cotton (Gossypium hirsutum L.) for use in high- and low-throughput molecular applications. This method is performed at room... Fri, 11 Jan 2013 18:41:06 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/January/Separation-of-integument-and-nucellar-tissues-from-cotton-ovules-Gossypium-hirsutum-L.-for-both-high--and-low-throughput-molecular-applications/biotechniques-338247.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Genome-engineering-methods-and-applications/biotechniques-338102.html Tue, 11 Dec 2012 19:25:03 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Genome-engineering-methods-and-applications/biotechniques-338102.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Quality-control-in-multi-tag-pyrosequencing-of-microbial-communities/biotechniques-338101.html Multi-tag pyrosequencing has become a key method in the analysis of microbial community composition. However, it is well known that kinetic bias during the initial PCR amplification of such microbial communities can dramatically distort amplicon... Mon, 10 Dec 2012 21:30:08 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Quality-control-in-multi-tag-pyrosequencing-of-microbial-communities/biotechniques-338101.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Hot-wiring-cells/biotechniques-338098.html Tue, 11 Dec 2012 19:25:02 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Hot-wiring-cells/biotechniques-338098.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Directed-evolution-of-nucleotide-based-libraries-using-lambda-exonuclease/biotechniques-338096.html Directed evolution of nucleotide libraries using recombination or mutagenesis is an important technique for customizing catalytic or biophysical traits of proteins. Conventional directed evolution methods, however, suffer from cumbersome digestion and... Tue, 11 Dec 2012 19:12:04 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Directed-evolution-of-nucleotide-based-libraries-using-lambda-exonuclease/biotechniques-338096.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Direct-sequencing-of-small-genomes-on-the-Pacific-Biosciences-RS-without-library-preparation/biotechniques-338089.html We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and... Mon, 10 Dec 2012 21:14:07 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Direct-sequencing-of-small-genomes-on-the-Pacific-Biosciences-RS-without-library-preparation/biotechniques-338089.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Colony-PCR/biotechniques-338083.html Mon, 10 Dec 2012 21:10:02 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Colony-PCR/biotechniques-338083.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Pimp-my-spec/biotechniques-338081.html Mon, 10 Dec 2012 21:05:07 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Pimp-my-spec/biotechniques-338081.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Profile-of-Piotr-Chomczynski/biotechniques-338079.html Mon, 10 Dec 2012 20:59:02 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Profile-of-Piotr-Chomczynski/biotechniques-338079.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Citations/biotechniques-338077.html Mon, 10 Dec 2012 20:52:03 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Citations/biotechniques-338077.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/BioSpotlight/biotechniques-338073.html Mon, 10 Dec 2012 20:45:02 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/BioSpotlight/biotechniques-338073.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/Our-annual-meeting/biotechniques-338070.html Mon, 10 Dec 2012 20:40:01 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/Our-annual-meeting/biotechniques-338070.html http://www.biotechniques.com/BiotechniquesJournal/2012/December/cDNA-normalization-by-hydroxyapatite-chromatography-to-enrich-transcriptome-diversity-in-RNA-seq-applications/biotechniques-338067.html Second-generation sequencing (SGS) has become the preferred method for RNA transcriptome profiling of organisms and single cells. However, SGS analysis of transcriptome diversity (including protein-coding transcripts and regulatory non-coding RNAs) is... Mon, 10 Dec 2012 20:32:14 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/December/cDNA-normalization-by-hydroxyapatite-chromatography-to-enrich-transcriptome-diversity-in-RNA-seq-applications/biotechniques-338067.html http://www.biotechniques.com/BiotechniquesJournal/2012/November/prfectBLAST-a-platform-independent-portable-front-end-for-the-command-terminal-BLAST-stand-alone-suite/biotechniques-337104.html prfectBLAST is a multiplatform graphical user interface (GUI) for the stand-alone BLAST+ suite of applications. It allows researchers to do nucleotide or amino acid sequence similarity searches against public (or user-customized) databases that are... Mon, 12 Nov 2012 20:20:02 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/November/prfectBLAST-a-platform-independent-portable-front-end-for-the-command-terminal-BLAST-stand-alone-suite/biotechniques-337104.html http://www.biotechniques.com/BiotechniquesJournal/2012/November/Immunohistochemistry-background-staining/biotechniques-337101.html Mon, 12 Nov 2012 20:15:01 +0000 http://www.biotechniques.com/BiotechniquesJournal/2012/November/Immunohistochemistry-background-staining/biotechniques-337101.html