http://www.biotechniques.com/BiotechniquesJournal/ In the Journal en-us ℗ & © 2013 of Informa Pharma. Please refer to the Terms and Conditions of Use at http://www.biotechniques.com rss@biotechniques.com Wed, 12 Jun 2013 19:57:18 +0100 Wed, 19 Jun 2013 12:06:11 +0100 60 In the Journal http://www.biotechniques.com/BiotechniquesJournal/2013/June/Capturing-protein-coding-genes-across-highly-divergent-species/biotechniques-344030.html DNA hybridization capture combined with next generation sequencing can be used to determine the sequences of hundreds of target genes across hundreds of individuals in a single experiment. However, the approach has thus far only been... Wed, 12 Jun 2013 19:57:18 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Capturing-protein-coding-genes-across-highly-divergent-species/biotechniques-344030.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Continuous-Fluorescence-Monitoring-of-Rapid-Cycle-DNA-Amplification/biotechniques-343955.html Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR Green I, (ii) a decrease in fluorescein quenching by rhodamine after... Tue, 11 Jun 2013 16:46:58 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Continuous-Fluorescence-Monitoring-of-Rapid-Cycle-DNA-Amplification/biotechniques-343955.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Making-it-Real-Time/biotechniques-343950.html It has been 30 years since the first description of the polymerase chain reaction (PCR)&#045;corresponding, coincidentally, with the year of the first issue of BioTechniques. Ensuing decades have seen remarkable advances in this revolutionary... Tue, 18 Jun 2013 17:10:37 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Making-it-Real-Time/biotechniques-343950.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Development-of-a-high-density-hemagglutinin-protein-microarray-to-determine----the-breadth-of-influenza-antibody-responses/biotechniques-343946.html We have developed an influenza hemagglutinin protein microarray to assess humoral recognition of diverse influenza strains induced by vaccination and infection. Each array consists of controls and 127 hemagglutinin antigens from 60 viruses,... Tue, 18 Jun 2013 17:08:49 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Development-of-a-high-density-hemagglutinin-protein-microarray-to-determine----the-breadth-of-influenza-antibody-responses/biotechniques-343946.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Players-in-the-beautiful-history-of-PCR/biotechniques-343936.html Tue, 18 Jun 2013 17:14:17 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Players-in-the-beautiful-history-of-PCR/biotechniques-343936.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Live-cell-imaging/biotechniques-343935.html Mon, 10 Jun 2013 21:29:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Live-cell-imaging/biotechniques-343935.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Visiting-Noncodarnia/biotechniques-343933.html Mon, 10 Jun 2013 20:59:05 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Visiting-Noncodarnia/biotechniques-343933.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Profile-of-Joe-Tsien/biotechniques-343931.html Mon, 10 Jun 2013 20:50:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Profile-of-Joe-Tsien/biotechniques-343931.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Citations/biotechniques-343929.html Mon, 10 Jun 2013 20:39:04 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Citations/biotechniques-343929.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/BioSpotlight/biotechniques-343927.html Mon, 10 Jun 2013 20:25:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/BioSpotlight/biotechniques-343927.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Trends-and-Traditions/biotechniques-343924.html Mon, 10 Jun 2013 20:09:01 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Trends-and-Traditions/biotechniques-343924.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Digital-PCR-to-determine-the-number-of-transcripts-from-single-neurons-after----patch-clamp-recording/biotechniques-343922.html Whole-cell patch-clamp recording enables detection of electrophysiological signals from single neurons as well as harvesting of perisomatic RNA through the patch pipette for subsequent gene expression analysis. Amplification and profiling of RNA... Tue, 18 Jun 2013 14:56:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Digital-PCR-to-determine-the-number-of-transcripts-from-single-neurons-after----patch-clamp-recording/biotechniques-343922.html http://www.biotechniques.com/BiotechniquesJournal/2013/June/Production-of-single-stranded-DNAs-by-self-cleavage-of-rolling-circle----amplification-products/biotechniques-343910.html DNA molecules that encode a small, high-speed self-hydrolyzing deoxyribozyme are used as templates for rolling circle amplification (RCA) to produce single-stranded DNAs (ssDNAs) of single- and multiple-unit lengths. Including self-cleaving... Tue, 18 Jun 2013 17:06:26 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/June/Production-of-single-stranded-DNAs-by-self-cleavage-of-rolling-circle----amplification-products/biotechniques-343910.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Lane-by-lane-sequencing-using-Illuminas-Genome-Analyzer-II/biotechniques-343075.html Next-generation sequencing has become an essential tool in molecular biology that has been successfully applied to a broad variety of experimental approaches. While several platforms for next-generation sequencing exist, the most commonly used approach... Wed, 15 May 2013 19:31:48 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Lane-by-lane-sequencing-using-Illuminas-Genome-Analyzer-II/biotechniques-343075.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/A-convenient-and-efficient-purification-method-for-chemically-labeled-oligonucleotides/biotechniques-343071.html We developed an efficient, cost-effective, and rapid purification method for chemically-labeled oligonucleotides that requires less time than conventional procedures such as ethanol precipitation or size-exclusion chromatography. Based on the hydrophilic... Tue, 14 May 2013 20:08:01 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/A-convenient-and-efficient-purification-method-for-chemically-labeled-oligonucleotides/biotechniques-343071.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Quantifying-antibody-binding-on-protein-microarrays-using-microarray-nonlinear-calibration/biotechniques-343066.html We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A... Wed, 15 May 2013 19:29:53 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Quantifying-antibody-binding-on-protein-microarrays-using-microarray-nonlinear-calibration/biotechniques-343066.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Pairwise-agonist-scanning-flow-cytometry-PAS-FC-measures-inside-out-signaling-and-patient-specific-response-to-combinatorial-platelet-agonists/biotechniques-343056.html <abstract><p>Understanding the response of cells to multiple stimuli is vital for predicting donor specific responses and better understanding the signaling pathways involved. This is of particular importance in platelets because exposure of... Fri, 10 May 2013 18:39:12 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Pairwise-agonist-scanning-flow-cytometry-PAS-FC-measures-inside-out-signaling-and-patient-specific-response-to-combinatorial-platelet-agonists/biotechniques-343056.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/MetaboQuant-a-tool-combining-individual-peak-calibration-and-outlier-detection-for-accurate-metabolite-quantification-in-1D-1H-and-1H-13C-HSQC-NMR-spectra/biotechniques-343053.html Solution nuclear magnetic resonance (NMR) spectroscopy is widely used to analyze complex mixtures of organic compounds such as biological fluids and tissue extracts. Targeted profiling approaches with reliable compound quantitifcation are hampered,... Tue, 14 May 2013 20:10:11 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/MetaboQuant-a-tool-combining-individual-peak-calibration-and-outlier-detection-for-accurate-metabolite-quantification-in-1D-1H-and-1H-13C-HSQC-NMR-spectra/biotechniques-343053.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Primer-design/biotechniques-343049.html Fri, 10 May 2013 17:49:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Primer-design/biotechniques-343049.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-chemist-in-the-kitchen/biotechniques-343047.html Fri, 10 May 2013 17:43:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-chemist-in-the-kitchen/biotechniques-343047.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Profile-of-Bruce-Beutler/biotechniques-343045.html Fri, 10 May 2013 17:36:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Profile-of-Bruce-Beutler/biotechniques-343045.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Citations/biotechniques-343043.html Fri, 10 May 2013 17:31:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Citations/biotechniques-343043.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/BioSpotlight/biotechniques-343041.html Fri, 10 May 2013 17:28:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/BioSpotlight/biotechniques-343041.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-Write-Way/biotechniques-343038.html Fri, 10 May 2013 17:23:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-Write-Way/biotechniques-343038.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/The-Importance-of-Cell-Line-Authentication/biotechniques-342108.html Fri, 12 Apr 2013 20:18:01 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/The-Importance-of-Cell-Line-Authentication/biotechniques-342108.html