http://www.biotechniques.com/BiotechniquesJournal/ In the Journal en-us ℗ & © 2013 of Informa Pharma. Please refer to the Terms and Conditions of Use at http://www.biotechniques.com rss@biotechniques.com Wed, 15 May 2013 19:31:48 +0100 Sun, 19 May 2013 02:16:39 +0100 60 In the Journal http://www.biotechniques.com/BiotechniquesJournal/2013/May/Lane-by-lane-sequencing-using-Illuminas-Genome-Analyzer-II/biotechniques-343075.html Next-generation sequencing has become an essential tool in molecular biology that has been successfully applied to a broad variety of experimental approaches. While several platforms for next-generation sequencing exist, the most commonly used approach... Wed, 15 May 2013 19:31:48 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Lane-by-lane-sequencing-using-Illuminas-Genome-Analyzer-II/biotechniques-343075.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/A-convenient-and-efficient-purification-method-for-chemically-labeled-oligonucleotides/biotechniques-343071.html We developed an efficient, cost-effective, and rapid purification method for chemically-labeled oligonucleotides that requires less time than conventional procedures such as ethanol precipitation or size-exclusion chromatography. Based on the hydrophilic... Tue, 14 May 2013 20:08:01 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/A-convenient-and-efficient-purification-method-for-chemically-labeled-oligonucleotides/biotechniques-343071.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Quantifying-antibody-binding-on-protein-microarrays-using-microarray-nonlinear-calibration/biotechniques-343066.html We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A... Wed, 15 May 2013 19:29:53 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Quantifying-antibody-binding-on-protein-microarrays-using-microarray-nonlinear-calibration/biotechniques-343066.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Pairwise-agonist-scanning-flow-cytometry-PAS-FC-measures-inside-out-signaling-and-patient-specific-response-to-combinatorial-platelet-agonists/biotechniques-343056.html <abstract><p>Understanding the response of cells to multiple stimuli is vital for predicting donor specific responses and better understanding the signaling pathways involved. This is of particular importance in platelets because exposure of... Fri, 10 May 2013 18:39:12 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Pairwise-agonist-scanning-flow-cytometry-PAS-FC-measures-inside-out-signaling-and-patient-specific-response-to-combinatorial-platelet-agonists/biotechniques-343056.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/MetaboQuant-a-tool-combining-individual-peak-calibration-and-outlier-detection-for-accurate-metabolite-quantification-in-1D-1H-and-1H-13C-HSQC-NMR-spectra/biotechniques-343053.html Solution nuclear magnetic resonance (NMR) spectroscopy is widely used to analyze complex mixtures of organic compounds such as biological fluids and tissue extracts. Targeted profiling approaches with reliable compound quantitifcation are hampered,... Tue, 14 May 2013 20:10:11 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/MetaboQuant-a-tool-combining-individual-peak-calibration-and-outlier-detection-for-accurate-metabolite-quantification-in-1D-1H-and-1H-13C-HSQC-NMR-spectra/biotechniques-343053.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Primer-design/biotechniques-343049.html Fri, 10 May 2013 17:49:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Primer-design/biotechniques-343049.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-chemist-in-the-kitchen/biotechniques-343047.html Fri, 10 May 2013 17:43:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-chemist-in-the-kitchen/biotechniques-343047.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Profile-of-Bruce-Beutler/biotechniques-343045.html Fri, 10 May 2013 17:36:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Profile-of-Bruce-Beutler/biotechniques-343045.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/Citations/biotechniques-343043.html Fri, 10 May 2013 17:31:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/Citations/biotechniques-343043.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/BioSpotlight/biotechniques-343041.html Fri, 10 May 2013 17:28:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/BioSpotlight/biotechniques-343041.html http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-Write-Way/biotechniques-343038.html Fri, 10 May 2013 17:23:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/May/The-Write-Way/biotechniques-343038.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/The-Importance-of-Cell-Line-Authentication/biotechniques-342108.html Fri, 12 Apr 2013 20:18:01 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/The-Importance-of-Cell-Line-Authentication/biotechniques-342108.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/A-simple-method-to-generate-stable-cell-lines-for-the-analysis-of-transient-protein-protein-interactions/biotechniques-342107.html Transient protein-protein interactions form the basis of signal transduction pathways in addition to many other biological processes. One tool for studying these interactions is bioluminescence resonance energ y transfer (BRET). This technique has been... Fri, 12 Apr 2013 20:10:08 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/A-simple-method-to-generate-stable-cell-lines-for-the-analysis-of-transient-protein-protein-interactions/biotechniques-342107.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/Improved-Native-Isolation-of-Endogenous-Protein-A-Tagged-Protein-Complexes/biotechniques-342101.html Here we report a modified peptide reagent useful for the rapid, native elution of protein complexes containing a Protein-A-tagged component. We tested this reagent for the elution of tagged endogenous protein complexes from yeast (Nup53p/Nup170p... Fri, 26 Apr 2013 19:31:48 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/Improved-Native-Isolation-of-Endogenous-Protein-A-Tagged-Protein-Complexes/biotechniques-342101.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/5-RACE/biotechniques-342096.html Fri, 12 Apr 2013 19:51:01 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/5-RACE/biotechniques-342096.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/A-designed-phase-changing-RTX-based-peptide-for-efficient-bioseparations/biotechniques-342093.html Typically, chromatography is the most costly and time-consuming step in protein purification. As a result, alternative methods have been sought for bioseparations, including the use of stimulus-responsive tags that can reversibly precipitate out... Fri, 26 Apr 2013 15:56:43 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/A-designed-phase-changing-RTX-based-peptide-for-efficient-bioseparations/biotechniques-342093.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/The-New-Genetic-Engineering-Toolbox/biotechniques-342087.html Fri, 12 Apr 2013 19:33:07 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/The-New-Genetic-Engineering-Toolbox/biotechniques-342087.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/Profile-of-Paul-Soloway/biotechniques-342085.html Fri, 12 Apr 2013 19:28:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/Profile-of-Paul-Soloway/biotechniques-342085.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/Citations/biotechniques-342083.html Fri, 12 Apr 2013 19:23:03 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/Citations/biotechniques-342083.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/BioSpotlight/biotechniques-342080.html Fri, 12 Apr 2013 19:10:02 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/BioSpotlight/biotechniques-342080.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/Expression-analysis-of-rare-cellular-subsets-direct-RT-PCR-on-limited-cell-numbers-obtained-by-FACS-or-soft-agar-assays/biotechniques-342077.html Since tissues and tumors are heterogenous populations containing different cell types, their transcriptomes are blends of multiple mRNA expression profiles. Although fluorescence-activated cell sorting (FACS) allows isolation of individual cell types,... Fri, 03 May 2013 15:14:05 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/Expression-analysis-of-rare-cellular-subsets-direct-RT-PCR-on-limited-cell-numbers-obtained-by-FACS-or-soft-agar-assays/biotechniques-342077.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/A-cell-free-assay-to-determine-the-stoichiometry-of-plasma-membrane-proteins/biotechniques-342069.html Plasma membrane receptors, transporters, and ion channel molecules are often found as oligomeric structures that participate in signaling cascades essential for cell survival. Different states of protein oligomerization may play a role in... Fri, 12 Apr 2013 21:40:24 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/A-cell-free-assay-to-determine-the-stoichiometry-of-plasma-membrane-proteins/biotechniques-342069.html http://www.biotechniques.com/BiotechniquesJournal/2013/April/Optimized-Blocking-Of-Porous-Nitrocellulose-Films-For-Sensitive-Protein-Microarrays/biotechniques-342064.html AbstractSuper G Blocking Buffer was developed to produce low backgrounds in assays using nitrocellulose film-slides. Using this blocking reagent in combination with ONCYTE&#174; SuperNOVA Film-Slides, we achieved an order of magnitude greater sensitivity... Fri, 12 Apr 2013 18:36:08 +0100 http://www.biotechniques.com/BiotechniquesJournal/2013/April/Optimized-Blocking-Of-Porous-Nitrocellulose-Films-For-Sensitive-Protein-Microarrays/biotechniques-342064.html http://www.biotechniques.com/BiotechniquesJournal/2013/March/Impact-of-H2O2-Decontamination-Technology-Over-High-Heat-Sterilization-Increased-Efficiency-and-Efficacy-with-Significantly-Reduced-Downtime/biotechniques-341106.html Tue, 12 Mar 2013 20:46:04 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/March/Impact-of-H2O2-Decontamination-Technology-Over-High-Heat-Sterilization-Increased-Efficiency-and-Efficacy-with-Significantly-Reduced-Downtime/biotechniques-341106.html http://www.biotechniques.com/BiotechniquesJournal/2013/March/Streamlining-the-Genotyping-Workflow-with-the-MultiGene-OptiMax/biotechniques-341103.html Tue, 12 Mar 2013 20:38:09 +0000 http://www.biotechniques.com/BiotechniquesJournal/2013/March/Streamlining-the-Genotyping-Workflow-with-the-MultiGene-OptiMax/biotechniques-341103.html