Advanced Online Publication
Characterization of human mitochondrial genome sequences is important for the molecular diagnosis of mitochondrial diseases, especially in samples with a low level of mitochondrial DNA (mtDNA) heteroplasmy (≥5%).
Sha Tang,
and Taosheng Huang
For cellular and animal transgenesis, FLP-and Cre-recombinase gene capture systems are highly effective to provide stable integration of a donor plasmid carrying the transgene cassette of interest into an engineered genomic locus in a given cell line.
Jannik E. Jakobsen,
Jacob G. Mikkelsen,
and Anders L. Nielsen
Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats.
Aron J. Fazekas,
Royce Steeves,
and Steven G. Newmaster
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