Advanced Online Publication
RNA interference (RNAi) is a commonly used technique for reverse genetic approaches in Caenorhabditis elegans. Feeding RNAi is the most convenient and inexpensive method for performing genome-wide RNAi screens.
Kyoengwoo Min
Human cancer cell lines and xenografts are valuable samples for whole-genome sequencing of human cancer. Tumors can be maintained by serial xenografting in athymic (nude) or severe combined immunodeficient (SCID) mice.
Ming-Tseh Lin,
Li-Hui Tseng,
Hirohiko Kamiyama,
Mihoko Kamiyama,
Phillip Lim,
Manuel Hidalgo,
Sarah Wheelan
DNA extraction is the first step in many molecular biology protocols. However, we hypothesized that DNA from a preserved specimen can leak into its preservative medium, allowing the medium itself to be directly PCR amplified.
Shadi Shokralla,
Gregory A. C. Singer,
and Mehrdad Hajibabaei
The development of small molecule and antibody inhibitors targeting the interaction of receptor tyrosine kinases (RTKs), such as epidermal growth factor receptor (EGFR), is of high pharmacological and biological interest.
Aaron Gajadhar,
and Abhijit Guha
Three-dimensional collagen gel contraction is the standard assay utilized for functionally quantifying a variety of cell types, in particular smooth muscle cells (SMCs) and myofibroblasts.
Roger M. Ilagan,
Kelly I. Guthrie,
Sarah F. Quinlan,
H. Scott Rapoport,
Sarah Jones,
Ashley Church,
Joydeep Basu,
and John W. Ludlow
Fluorescence imaging in plants is unusually challenging because of the large amounts of photosynthetic pigments contained in green plant tissues.
Lu Zhang,
Klaus Gase,
Ian T. Baldwin,
and Ivan Gális
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