BioTechniques for Preclinical Development | September 2009
Volume:47
, Issue Number:3
Cover Image:
Epidermal growth factor receptor (EGFR) gene–protein triple staining on formalin-fixed, paraffin-embedded human epithelial carcinoma A431 cell line xenograft tumor. Blue staining indicates EGFR protein by alkaline phosphatase (AP)-based immunohistochemical detection. Black dots demonstrate amplified EGFR gene copies in the nuclei by horseradish peroxidase (HRP)–based silver in situ hybridization (SISH) detection. Red dots demonstrate chromosome 7 centromere (EGFR gene is on chromosome 7) by AP-based chromogenic in situ hybridization (CISH) detection. Image courtesy of Hiro Nitta and Thomas M. Grogan, Office of Medical Affairs, Ventana Medical Systems, Inc., Tucson, AZ, USA.
Short Technical Reports
Derek S. Reubish,
Daniel E. Emerling,
Jeff DeFalco,
Daniel Steiger,
Cheryl L. Victoria,
and Fabien Vincent
BioTechniques, Vol. 47, No. 3, September 2009, pp. iii–ix
doi
10.2144/000113198
Nezamoddin N. Kachouie,
Lifeng Kang,
and Ali Khademhosseini
BioTechniques, Vol. 47, No. 3, September 2009, pp. x–xvi
doi
10.2144/000113202