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Quantification of intracellular proteins and phosphorylation events is
extremely important for biomedical research. Although Western blot is the
most widely used method, it is labor intensive and time-consuming,
especially when analyzing multiple samples.
To address the bottlenecks in over-expression and purification of soluble and functional proteins, we have developed HaloTag®, a 34 kDa, monomeric derivative of dehalogenase, engineered to enhance expression and solubility of recombinant proteins in E. coli and to provide efficient protein purification coupled with tag removal.
The Pressure Cycling Technology Sample Preparation System (“PCT SPS”) employs
rapid cycles of hydrostatic pressure, between ambient and ultra high levels,
to precisely control biomolecular interactions. The PCT SPS can be used to
accelerate enzymatic reactions, such as protein digestion with trypsin and
other proteolytic enzymes, to prepare samples for analysis by mass
Primary cells as well as certain cell-lines are known to be refractory to traditional chemical transfection methods. The physical technique of electroporation has emerged as a method of choice for nucleic acid delivery into these “hard to transfect” cells.
Human cell lines and tumors represent important tools in biomedical research and they are extensively used. The most common sources of human cell lines and tumors are specimens taken directly from patients and cultured specimens stored and distributed by repositories and cell banks.
Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and has a substantial negative impact on biomedical research. In this study, an approach used by RADIL for analyzing human and rodent cell lines for inter and intra--species contamination followed by authentication of cell line identity is described.