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The Transplex® Complete Whole Transcriptome Amplification (WTA2) Kit has been
enhanced to effectively amplify damaged RNA and low input amounts of
RNA.Total RNA samples isolated from matched
FFPE(formalin-fixedparaffin-embedded) and frozen prostate tissues (malignant
and normal) were amplified with the Transplex® Complete WTA2 Kit and
analyzed on Agilent Whole Genome expression microarrays. A call rate of >65%
was observed for combined FFPE and frozen expression differentials
(Malignant/Normal), with >90% commonality between the FFPE and frozen
Quantification of intracellular proteins and phosphorylation events is
extremely important for biomedical research. Although Western blot is the
most widely used method, it is labor intensive and time-consuming,
especially when analyzing multiple samples.
To address the bottlenecks in over-expression and purification of soluble and functional proteins, we have developed HaloTag®, a 34 kDa, monomeric derivative of dehalogenase, engineered to enhance expression and solubility of recombinant proteins in E. coli and to provide efficient protein purification coupled with tag removal.
The Pressure Cycling Technology Sample Preparation System (“PCT SPS”) employs
rapid cycles of hydrostatic pressure, between ambient and ultra high levels,
to precisely control biomolecular interactions. The PCT SPS can be used to
accelerate enzymatic reactions, such as protein digestion with trypsin and
other proteolytic enzymes, to prepare samples for analysis by mass
Primary cells as well as certain cell-lines are known to be refractory to traditional chemical transfection methods. The physical technique of electroporation has emerged as a method of choice for nucleic acid delivery into these “hard to transfect” cells.
Human cell lines and tumors represent important tools in biomedical research and they are extensively used. The most common sources of human cell lines and tumors are specimens taken directly from patients and cultured specimens stored and distributed by repositories and cell banks.