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ArthritoMab™ Antibody Cocktail: a cocktail of anti-collagen type II antibodies induce a synchronized model of Arthritis in just 12 days.
Gordon Meiklejohn, PhD., Michelle Ierna PhD., Hannah Scales, PhD, Eric Benson, PhD., Holly Olso
MD Biosciences, Inc.
Sponsored by MD Biosciences, Inc
Published in December 2009 (p.3) DOI:  10.2144/000113334 Sponsored,vendor-submitted protocol
The CIA model is mediated by autoantibodies, which bind to a particular region of type II collagen (CII) and complement. Research has shown an antibody cocktail of collagen type II antibodies selected for their epitope specificity can induce arthritis. Evidence suggests that an antibody response to certain epitopes is better associated with arthritis than other epitopes. In the CIA mouse model, the antibody response that correlated with arthritis was mainly associated with the bind- ing to the epitopes C1, J1 and U1.
MD Biosciences ArthritoMab™ Antibody cocktail consists of four monoclo- nal antibodies to CII, 3 of which are IgG2b isotypes which induce stronger arthritis. These antibodies bind to the well-defined epitopes C11b, J1, D3 and U1, which are all major epitopes in mice immunized with CII and develop- ing arthritis. These epitopes are also spread across the entire CII region (CB8, CB10, and CB11 fragments) possibly encouraging better immune complex formation on the cartilage surface or in the synovium. These complexes can then activate complement and induce inflammation by both the classical and alternative pathways. Monocytes in the joint are also activated by these complexes via Fc receptors releasing pro-inflammatory cytokines (i.e. TNF-α, IL-1β) that recruit neutrophils and macrophages.
The ability to induce arthritis using an antibody cocktail to CII provides an efficient protocol as an alternative to the lengthy CIA protocol.
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