to BioTechniques free email alert service to receive content updates.
POSTER
A Competitive, Colorimetric ELISA for Quantification of 24(S)-Hydroxycholesterol, a Biomarker of Neurodegeneration
Dominique Wilson, Rory Olson, Mike Mullenix
Enzo Life Sciences, Farmingdale, NY 11735, USA
Sponsored by Enzo Life Sciences
Enzo Life Sciences, Farmingdale, NY 11735, USA
Sponsored by Enzo Life Sciences
Cholesterol imbalance is implicated in a variety of neurodegenerative diseases including Alzheimer’s Disease, Huntington’s Disease, and Multiple Sclerosis. Excess cholesterol is removed from the brain via the hydroxylation of C24 on cholesterol by CYP46 enzyme, a highly conserved member of the cytochrome P450 family, to the more soluble 24(S)-Hydroxycholesterol (24-OHC) which is able to cross the blood brain barrier. Levels of 24-OHC in the cerebral spinal fluid (CSF) may give insight into the homeostasis of cholesterol within the central nervous system, and may serve as a valuable biomarker for neurodegenerative disease. We have developed a competitive colorimetric ELISA for the measurement of 24-OHC that can be used in both in vivo and in vitro studies. The assay is highly specific to 24-OHC, exhibiting less than 0.02% cross-reactivity to cholesterol, 22-hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol and dehydroepiandrosterone (DHEA). The assay returns 24-OHC levels in CSF consistent with levels reported in the literature, and is also suitable for tissue homogenates and tissue culture supernatants, requiring 100 L or less of sample volume. The assay has a dynamic range from 0.78 to 100 ng/mL and a time to answer of 2 hours, making it a convenient alternative to more labor- and instrument-intensive methods such as GC-MS, LC-MS and HPLC-MS.
More Information
Do you have question about this Poster? Would you like more information about this poster?
Submit your questions here and BioTechniques will forward your question and contact information from our
database to an expert at
Enzo Life Sciences
Contact Information
Enzo Life Sciences
Farmingdale
NY
11735
USA
Tel:1-800-942-0430
Fax:1-610-941-9252
E-mail:
[email protected]
Poster Categories
