Jamie L. Hale, Michele M. Bush, Harold Baker, Michaela R. Hoffmeyer
Luminex Corporation, Austin, Texas
Detection of emerging or acute infections of Avian Influenza (AI), Mycoplasma synoviae (MS), and Mycoplasma gallisepticum (MG) is critical to prevent dramatic loss to the flock, dictate appropriate treatment measures, and limit disease spread. Commercial producers monitor flocks regularly for AI, MS, or MG infection, typically by ELISA or gold standard methods. However, testing for response to multiple pathogens can be laborious, as each pathogen requires a separate test and sample. Furthermore, such tests can be difficult to automate and prone to experimental error. We have developed a multiplexed magnetic microsphere-based assay that will diagnose infection with AI, MS, and MG simultaneously in about 2.5 hours. Different color microsphere regions are covalently coated with inactivated pathogen-derived antigen and generate consistent results lot to lot. The magnetic microspheres allow for easy automation and facilitate washing steps which may benefit high throughput laboratories. Internal control microsphere regions control for sample addition, detection reagent addition, and nonspecific binding. Results from reference panel samples acquired from NVSL and the University of Georgia correlate to expected results and precision within and between runs is excellent.
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