Improved Cultivation and Differentiation of Embryonic Stem Cells
Greiner Bio-One GmbH, Maybachstr. 2, D-72636 Frickenhausen, Germany, www.gbo.com/bioscience
Embryonic stem cells (ESCs) have gained considerable interest in recent years due to their capacity to both self-renew and differentiate into cells of all three germ layers. They can develop into any type of cell or tissue in the body and are especially attractive for regenerative therapies. Widespread use of ESCs for therapeutic approaches has been hampered by the complexity of the conditions required for their maintenance and differentiation. Therefore scientists try to establish protocols which reliably direct the differentiation of embryonic stem cells into specific cell types. In the presented set of experiments, we show that murine embryonic stem cells can be cultivated on Greiner Bio-One surfaces keeping them in an undifferentiated state. Furthermore, we present a simple though efficient protocol for the differentiation of ESCs into neurons.