Microscope-based, high content instruments are used for many cell based assays in high content screening (HCS). For efficient and rapid analysis most assays require the use of higher resolutions which entail lengthy read times, using single colours.
Accurate determination of molecular concentrations is a prerequisite to the use of purified biomolecules for a multitude of downstream applications. Quantification is routinely accomplished by spectrophotometric analysis.
Cholesterol imbalance is implicated in a variety of neurodegenerative diseases including Alzheimer’s Disease, Huntington’s Disease, and Multiple Sclerosis. Excess cholesterol is removed from the brain via the hydroxylation of C24 on cholesterol by CYP46 enzyme, a highly conserved member of the cytochrome P450 family, to the more soluble 24(S)-Hydroxycholesterol (24-OHC) which is able to cross the blood brain barrier.
A large percentage of drugs fail in clinical studies,or are withdrawn from the market due to hepatic toxicity. Therefore, highly predictive in vitro assays suitable for safety and efficacy testing are extremely important for improving the drug development process and reducing drug attrition.
Embryonic stem cells (ESCs) have gained considerable interest in recent years due to their capacity to both self-renew and differentiate into cells of all three germ layers. They can develop into any type of cell or tissue in the body and are especially attractive for regenerative therapies.
Phenotypic loss-of-function RNAi screens with complex lentiviral-based shRNA expression libraries that target and silence several thousand genes provide a realistic and workable approach to identify genes that functionally modulate a cellular response such as viability of cancer cells or apoptosis. As long as the shRNA libraries are properly constructed so that hairpin representation is well characterized and reasonably constrained, and changes in shRNA representation in selected vs.
A major challenge facing the development of PI3K-targeted pharmaceutical agents is the lack of patient-relevant, in vitro model systems. Studies into the function of oncogenic mutations are commonly carried out in cell line panels that are mutant or wild-type for the gene of interest, however interpretation of these studies can be confounded by additional genetic differences between each cell line background, calling into question the reliability of the results.
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