Use of PowerPlex® 18D in the Examination of Genomic Instability in the Short Tandem Repeat DNA Loci used for Human Cell Line Authentication Testing
In this study, 76 human cancer cell lines, profiled using the commercially available PowerPlex® 18D multiplex amplification kit (Promega), were used to determine the feasibility of using STR DNA testing for cell line authentication and identification in the face of predicted genomic instability commonly found in cancer cell lines (changes at the chromosomal and nucleotide levels). Combined genomic instability was found in 26.4% of all cell lines tested.
We have recently developed a protein extraction and solubilization system for the extraction of “difficult” proteins from E. coli which allow for the recovery of proteins not previously recovered by standard extractioon buffers.
A system for detecting reference dyes in MicroTiter Plates and Array Tape for use as an in line process verification using the principals of standard addition. The system consists of hardware device, the fluorescent dyes and the samples to be tracked through a process.
A cell injection system designed to deliver small spatially-contained volumes while maintaining and promoting stem cell viability and functionality through controlled cooling
Small volume biological injections have traditionally been accomplished using thumb pressure on a hand-operated disposable syringe. Though this has been shown to be a workable situation in some cases, it has become clear that for protocol optimization, reproducibility, scale up and quantification, improved tools and methodology are required.
Microscope-based, high content instruments are used for many cell based assays in high content screening (HCS). For efficient and rapid analysis most assays require the use of higher resolutions which entail lengthy read times, using single colours.
Analytical Performance of a Higher Throughput Multi-Volume Microplate Accessory for Microplate Spectrophotometer Platforms
Accurate determination of molecular concentrations is a prerequisite to the use of purified biomolecules for a multitude of downstream applications. Quantification is routinely accomplished by spectrophotometric analysis.
A Competitive, Colorimetric ELISA for Quantification of 24(S)-Hydroxycholesterol, a Biomarker for Neurodegeneration 24(S)-Hydroxycholesterol (24-OHC) ELISA kit (ADI-900-210)
A Competitive, Colorimetric ELISA for Quantification of 24(S)-Hydroxycholesterol, a Biomarker of Neurodegeneration
Cholesterol imbalance is implicated in a variety of neurodegenerative diseases including Alzheimer’s Disease, Huntington’s Disease, and Multiple Sclerosis. Excess cholesterol is removed from the brain via the hydroxylation of C24 on cholesterol by CYP46 enzyme, a highly conserved member of the cytochrome P450 family, to the more soluble 24(S)-Hydroxycholesterol (24-OHC) which is able to cross the blood brain barrier.
Multiplexed High Content Assays for Predictive Hepatotoxicity using Induced Pluripotent Stem Cell Derived Hepatocytes
A large percentage of drugs fail in clinical studies,or are withdrawn from the market due to hepatic toxicity. Therefore, highly predictive in vitro assays suitable for safety and efficacy testing are extremely important for improving the drug development process and reducing drug attrition.
Cell and Tissue Analysis
Embryonic stem cells (ESCs) have gained considerable interest in recent years due to their capacity to both self-renew and differentiate into cells of all three germ layers. They can develop into any type of cell or tissue in the body and are especially attractive for regenerative therapies.