A suitable High-Throughput and High-Content Screening strategy has a major impact on drug discovery. The use of the most advanced technologies is a major prerequisite for successful screening.
Horizon Discovery X‐MAN™ cell lines reveal how PI3K mutation can result in EMT switch and increased invasiveness
A major challenge facing the development of PI3K-targeted pharmaceutical agents is the lack of patient-relevant, in vitro model systems. Studies into the function of oncogenic mutations are commonly carried out in cell line panels that are mutant or wild-type for the gene of interest, however interpretation of these studies can be confounded by additional genetic differences between each cell line background, calling into question the reliability of the results.
Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from ε-N-acetyl lysine amino acids on histones and other proteins. The removal of acetyl groups serves to increase the positive charge of histone tails, encouraging binding between histones and the DNA backbone, and preventing transcription.
Development of Homogeneous Non-radioactive Assays for Studying Histone H3 Methyltransferases and Demethylases
Post-translational modifications of histones are among the epigenetic mechanisms that can affect chromatin structure and function. Disruption of epigenetic processes can lead to altered gene expression and malignant cellular transformation.
Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases Targeting Histone H3K4, H3K27 and H3K36 Residues
Several assay methods have been developed for quantifying the activity of histone deacetylases (HDACs and sirtuins), histone methyltransferases (HMTs), and histone demethylases (HDMs). These include radioactive assays, enzymelinked immunoassays (ELISA), mass spectrometry, and enzyme-coupled detection of fluorescent peptides or reaction co-products (e.g.
Development of High-Throughput Assays to Study Histone H3K4 Methyltransferases & H3K9 Methyl- and Acetyltransferases
Post-translational modifications of histone proteins play an important part in a wide array of cellular processes including regulation of gene transcription, DNA repair, cell cycle, and metabolism control. For instance, transcriptional activation is associated with acetylation of Histone H3 on residues K9 and K14, and methylation on K4.