The Strep-tag II is ideal as an affinity tag due to the small size (8 aa, 1 kDa), often making removal of the tag unnecessary.
StrepTrap™ HP 1 ml and 5 ml columns have been specially developed for high specificity towards the Strep-tag II, with the prepacked format facilitating fast and convenient purification.
Protein kinases regulate many different signaling pathways and cellular processes. They have been implicated in various cancers, typically displaying increased activation, and are now being investigated as potential therapeutic targets.
Maltose binding protein (MBP) is beneficial as an affinity tag due to its ability to increase expression level and solubility of the fusion protein. The MBPTrap™ HP 1 ml and 5 ml columns have been specially developed for high specificity towards the MBP-tag and the prepacked format facilitate fast, convenient and reproducible purifications.
Gel filtration (GF) is an excellent tool to acquire information about sizing (identity), purity and specially the multimeric state of a protein of interest. Superdex™ 200 and Superdex 75 are supreme GF media for such analysis.
HisTrap FF crude, is developed for fast, simple and convenient purification of histidine-tagged proteins. Unclarified cell lysate can be applied directly on the column, without any centrifugation and filtration steps.
During tumorigenesis, cells acquire mutations that confer selective advantages for growth. These mutations may reprogram cellular metabolism to meet the requirements of rapid proliferation and survival in an environment of fluctuating and scarce nutrients.
Detection of emerging or acute infections of Avian Influenza (AI), Mycoplasma synoviae (MS), and Mycoplasma gallisepticum (MG) is critical to prevent dramatic loss to the flock, dictate appropriate treatment measures, and limit disease spread. Commercial producers monitor flocks regularly for AI, MS, or MG infection, typically by ELISA or gold standard methods.
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