Multiplexed Sandwich Assay for Quantitation of Transgenic Proteins in Genetically Modified Plants Using the Luminex® FLEXMAP 3D® System
Methods for testing transgene copy number and protein expression are necessary for development of genetically modified crops (GMO). Most transgenes impart herbicide resistance or protection from crop damaging insects and are proprietary.
Plasmid DNA Purification
Advances in cloning techniques have greatly increased the number of samples requiring small-scale plasmid preparation, or mini preps. The large scale purification schemes of the past have given way to the development of small scale, massively parallel purifications requiring semi-automated or fully-automated handling.
Advances in Next-Generation Sequencing (NGS) have increased both the throughput and capacity of sequencing platforms, calling for increased efficiency in sample preparation and the ability to work with small and precious samples. NuGEN’s portfolio of NGS products meets this challenge by enabling simple, rapid and affordable sample preparation workflows for key applications on leading NGS platforms.
The efficacy of amplification of small quantities of total RNA with the Transplex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasing numbers of FACS-isolated bone marrow stem cells(10-,100-,and1000-cell samples) was amplified with the Transplex® Complete WTA2 Kit.
Detection of Prostate Cancer BioMarkers by Expression Microarray Analysis of TransPlex® WTA2 Amplified FFPE Tissue RNA
The Transplex® Complete Whole Transcriptome Amplification (WTA2) Kit has been enhanced to effectively amplify damaged RNA and low input amounts of RNA.Total RNA samples isolated from matched FFPE(formalin-fixedparaffin-embedded) and frozen prostate tissues (malignant and normal) were amplified with the Transplex® Complete WTA2 Kit and analyzed on Agilent Whole Genome expression microarrays. A call rate of >65% was observed for combined FFPE and frozen expression differentials (Malignant/Normal), with >90% commonality between the FFPE and frozen datasets.
Development of a Novel Cell-Based ELISA for Analysis of Intracellular Proteins or Phosphorylation of Signaling Molecules.
Quantification of intracellular proteins and phosphorylation events is extremely important for biomedical research. Although Western blot is the most widely used method, it is labor intensive and time-consuming, especially when analyzing multiple samples.