Diana Caracino, David Onley and Paul Wylie
Microscope-based, high content instruments are used for many cell based assays in high content screening (HCS). For efficient and rapid analysis most assays require the use of higher resolutions which entail lengthy read times, using single colours. Furthermore, in order to keep plate read times at a minimum, often only a small percentage of the total number of cells are analyzed in a well. acumen® hci is a laser scanning imaging cytometer with a widefield objective lens and “on-the-fly” laser scanning capabilities. acumen rapidly scans entire wells, enabling statistically robust data to be obtained for a number of cell-based assays relevant to the drug discovery industry such as cell cycle analysis, cell signalling, cell surface marker expression and cell migration.
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