P. Brescia, G. Prescott, A. Rieger, K. Yarsargil, P. Banks
BioTek Instruments, Inc., Winooski, Vermont, USA
Accurate determination of molecular concentrations is a prerequisite to the use of purified biomolecules for a multitude of downstream applications. Quantification is routinely accomplished by spectrophotometric analysis. A novel microplate accessory is described here which incorporates the ability to directly measure up to forty-eight low-volume (2L) samples for direct quantification and two vertical 1 cm path length cells, in a standard microplate sized format. Its use allows a researcher to quantify samples without dilution while also allowing more dilute samples to be read in a standard 1 cm pathlength format. The analytical performance of the accessory will be discussed including limit of detection, dynamic range and accuracy for the quantification of total protein and isolated nucleic acids.
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