to BioTechniques free email alert service to receive content updates.

POSTER
Pressure-Enhanced Enzymatic Proteolysis
Sponsored by Pressure Biosciences Published in December 2009 (p.13) DOI:  10.2144/000113328
The Pressure Cycling Technology Sample Preparation System (“PCT SPS”) employs rapid cycles of hydrostatic pressure, between ambient and ultra high levels, to precisely control biomolecular interactions. The PCT SPS can be used to accelerate enzymatic reactions, such as protein digestion with trypsin and other proteolytic enzymes, to prepare samples for analysis by mass spectrometry. In addition, the PCT SPS can be used to disrupt tissues and cells to extract cellular structures and components such proteins, lipids, nucleic acids, and small molecules for further analysis. [1]. The PCT SPS is comprised of a Barocycler (a small, semiautomated bench-top instrument used to generate high hydrostatic pressure) and specially-designed, high pressure tolerant, single-use processing tubes. Temperature can be controlled during the PCT-based sample preparation process by an external circulating water bath, which can be an important addition to the Pressure Cycling Technology Sample Preparation System.
More Information
Do you have question about this Poster? Would you like more information about this poster?
Submit your questions here and BioTechniques will forward your question and contact information from our database to an expert at Pressure Biosciences
Request more information
Yes, please forward my question, along with my name, title, company, and e-mail address to Pressure Biosciences
BioTechniques will forward your question to the company, who will contact you directly by email.
 
Name Question
Job Title
Company
Email
Contact Information