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POSTER
Pressure-Enhanced Enzymatic Proteolysis
Sponsored by
Pressure Biosciences
Published in
December
2009
(p.13)
DOI: 10.2144/000113328
Published in
December
2009
(p.13)
DOI: 10.2144/000113328
The Pressure Cycling Technology Sample Preparation System (“PCT SPS”) employs
rapid cycles of hydrostatic pressure, between ambient and ultra high levels,
to precisely control biomolecular interactions. The PCT SPS can be used to
accelerate enzymatic reactions, such as protein digestion with trypsin and
other proteolytic enzymes, to prepare samples for analysis by mass
spectrometry. In addition, the PCT SPS can be used to disrupt tissues and
cells to extract cellular structures and components such proteins, lipids,
nucleic acids, and small molecules for further analysis. [1]. The PCT SPS is
comprised of a Barocycler (a small, semiautomated bench-top instrument used
to generate high hydrostatic pressure) and specially-designed, high pressure
tolerant, single-use processing tubes. Temperature can be controlled during
the PCT-based sample preparation process by an external circulating water
bath, which can be an important addition to the Pressure Cycling Technology
Sample Preparation System.
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