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Microarray Analysis
Posters
Ingenuity® iReport™ is a new web based interactive report that enables researchers to quickly understand gene expression data and identify biological insights backed by millions of published discoveries curated from the biomedical literature. We validated the ability of iReport to correctly identify expected results and gain novel biological insights by analyzing and interpreting previously published data from a study by Lee et al.
Expression Profiling
The Transplex® Complete Whole Transcriptome Amplification (WTA2) Kit has been enhanced to effectively amplify damaged RNA and low input amounts of RNA.Total RNA samples isolated from matched FFPE(formalin-fixedparaffin-embedded) and frozen prostate tissues (malignant and normal) were amplified with the Transplex® Complete WTA2 Kit and analyzed on Agilent Whole Genome expression microarrays. A call rate of >65% was observed for combined FFPE and frozen expression differentials (Malignant/Normal), with >90% commonality between the FFPE and frozen datasets.
Expression Profiling
The efficacy of amplification of small quantities of total RNA with the Transplex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasing numbers of FACS-isolated bone marrow stem cells(10-,100-,and1000-cell samples) was amplified with the Transplex® Complete WTA2 Kit.
Expression Profiling
The efficacy of amplification of small quantities of total RNA with the Transplex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasing numbers of FACS-isolated bone marrow stem cells(10-,100-,and1000-cell samples) was amplified with the Transplex® Complete WTA2 Kit.
Expression Profiling
The Transplex® Complete Whole Transcriptome Amplification (WTA2) Kit has been enhanced to effectively amplify damaged RNA and low input amounts of RNA.Total RNA samples isolated from matched FFPE(formalin-fixedparaffin-embedded) and frozen prostate tissues (malignant and normal) were amplified with the Transplex® Complete WTA2 Kit and analyzed on Agilent Whole Genome expression microarrays. A call rate of >65% was observed for combined FFPE and frozen expression differentials (Malignant/Normal), with >90% commonality between the FFPE and frozen datasets.