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Cell and tissue culture-stem cells
The human brain is a complex organ that has consistently been proven difficult to model in vitro. With current models suffering from various issues, including biological relevance, functionality, and reproducibility, a significant focus within the drug discovery field has been on the development and validation of more relevant in vitro human model systems.
Epigenomics
Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from ε-N-acetyl lysine amino acids on histones and other proteins. The removal of acetyl groups serves to increase the positive charge of histone tails, encouraging binding between histones and the DNA backbone, and preventing transcription.
Epigenetics
Post-translational modifications of histones are among the epigenetic mechanisms that can affect chromatin structure and function. Disruption of epigenetic processes can lead to altered gene expression and malignant cellular transformation.
Epigenetics
Several assay methods have been developed for quantifying the activity of histone deacetylases (HDACs and sirtuins), histone methyltransferases (HMTs), and histone demethylases (HDMs). These include radioactive assays, enzymelinked immunoassays (ELISA), mass spectrometry, and enzyme-coupled detection of fluorescent peptides or reaction co-products (e.g.
Epigenetics
Post-translational modifications of histone proteins play an important part in a wide array of cellular processes including regulation of gene transcription, DNA repair, cell cycle, and metabolism control. For instance, transcriptional activation is associated with acetylation of Histone H3 on residues K9 and K14, and methylation on K4.
Epigenetics
In eukaryotes, the covalent modification of histones has a crucial role in chromatin architecture and plays an important part in a plethora of cellular processes, from chromatin remodeling and transcriptional regulation, to DNA repair and cell cycle control. While histone acetylation is generally associated to an open chromatin state and transcriptional activation, methylation of histones has been related to either activating or repressive functions.
Mass Spectrometry
The PressureBioSciences Inc.(PBI) focuses on development of specialized products for biological sample preparation which utilize alternating ultra-high hydrostatic pressure–pressure cycling technology(PCT). PCT is a fundament althermo dynamic process which destabilizes molecular interactions by rapidly and repeatedly raising and lowering pressure in the action vessel from atmospheric to levels of up to 45,000 psi.
