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High Throughput
Protein Tyrosine Kinases (PTKs) play important roles in modulating a wide variety of cellular events, including differentiation, proliferation, metabolism and apoptosis. Those regulations are mediated by a series of signal cascades that involve phosphorylation of tyrosine residues in target proteins.
Transfection
Abstract: Transient transfection in mammalian cell lines provides an avenue
for researchers to bridge the development bottleneck and shorten the time to
usable protein. The method also maintains post translational modifications
crucial for biotherapeutic function.
Plasmid DNA Purification
Advances in cloning techniques have greatly increased the number of samples
requiring small-scale plasmid preparation, or mini preps. The large scale
purification schemes of the past have given way to the development of small
scale, massively parallel purifications requiring semi-automated or
fully-automated handling.
Sample Preparation
Advances in Next-Generation Sequencing (NGS) have increased both the throughput and capacity of sequencing platforms, calling for increased efficiency in sample preparation and the ability to work with small and precious samples. NuGEN’s portfolio of NGS products meets this challenge by enabling simple, rapid and affordable sample preparation workflows for key applications on leading NGS platforms.
Expression Profiling
The Transplex® Complete Whole Transcriptome Amplification (WTA2) Kit has been
enhanced to effectively amplify damaged RNA and low input amounts of
RNA.Total RNA samples isolated from matched
FFPE(formalin-fixedparaffin-embedded) and frozen prostate tissues (malignant
and normal) were amplified with the Transplex® Complete WTA2 Kit and
analyzed on Agilent Whole Genome expression microarrays. A call rate of >65%
was observed for combined FFPE and frozen expression differentials
(Malignant/Normal), with >90% commonality between the FFPE and frozen
datasets.
Expression Profiling
The efficacy of amplification of small quantities of total RNA with the
Transplex® Complete Whole Transcriptome Amplification Kit (WTA2) was
examined in this study. Total RNA extracted from decreasing numbers of
FACS-isolated bone marrow stem cells(10-,100-,and1000-cell samples) was
amplified with the Transplex® Complete WTA2 Kit.
High Throughput
Introduction: Array Tape is a continuous, flexible polymer serially embossed with reaction wells in customized volumes and formats. The Array Tape Platform includes modular liquid handling (Nexar), detection (Araya) and thermocycling (Soellex) instruments that increase throughput, flexibility and cost savings compared to microplate-based technologies.
High Throughput
The chemokine receptor CXCR4 has multiple critical functions in normal
physiologies including embryonic development of the cardiovascular,
hemopoietic and central nervous systems. It has also been implicated in
disease pathologies such as HIV infection, cancer metastasis, leukemia
progression and rheumatoid arthritis, and is fueling the search for small
molecule CXCR4 antagonists as a means for intervention1.
High Throughput
The chemokine receptor CXCR4 has multiple critical functions in normal
physiologies including embryonic development of the cardiovascular,
hemopoietic and central nervous systems. It has also been implicated in
disease pathologies such as HIV infection, cancer metastasis, leukemia
progression and rheumatoid arthritis, and is fueling the search for small
molecule CXCR4 antagonists as a means for intervention1.
