Methods for testing transgene copy number and protein expression are necessary for development of genetically modified crops (GMO). Most transgenes impart herbicide resistance or protection from crop damaging insects and are proprietary.
Advances in cloning techniques have greatly increased the number of samples requiring small-scale plasmid preparation, or mini preps. The large scale purification schemes of the past have given way to the development of small scale, massively parallel purifications requiring semi-automated or fully-automated handling.
Advances in Next-Generation Sequencing (NGS) have increased both the throughput and capacity of sequencing platforms, calling for increased efficiency in sample preparation and the ability to work with small and precious samples. NuGEN’s portfolio of NGS products meets this challenge by enabling simple, rapid and affordable sample preparation workflows for key applications on leading NGS platforms.
The efficacy of amplification of small quantities of total RNA with the
Transplex® Complete Whole Transcriptome Amplification Kit (WTA2) was
examined in this study. Total RNA extracted from decreasing numbers of
FACS-isolated bone marrow stem cells(10-,100-,and1000-cell samples) was
amplified with the Transplex® Complete WTA2 Kit.
The Transplex® Complete Whole Transcriptome Amplification (WTA2) Kit has been
enhanced to effectively amplify damaged RNA and low input amounts of
RNA.Total RNA samples isolated from matched
FFPE(formalin-fixedparaffin-embedded) and frozen prostate tissues (malignant
and normal) were amplified with the Transplex® Complete WTA2 Kit and
analyzed on Agilent Whole Genome expression microarrays. A call rate of >65%
was observed for combined FFPE and frozen expression differentials
(Malignant/Normal), with >90% commonality between the FFPE and frozen
Quantification of intracellular proteins and phosphorylation events is
extremely important for biomedical research. Although Western blot is the
most widely used method, it is labor intensive and time-consuming,
especially when analyzing multiple samples.
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