Development of a Novel Cell-Based ELISA for Analysis of Intracellular Proteins or Phosphorylation of Signaling Molecules.
Quantification of intracellular proteins and phosphorylation events is extremely important for biomedical research. Although Western blot is the most widely used method, it is labor intensive and time-consuming, especially when analyzing multiple samples.
Protein Isolation and Purification
To address the bottlenecks in over-expression and purification of soluble and functional proteins, we have developed HaloTag®, a 34 kDa, monomeric derivative of dehalogenase, engineered to enhance expression and solubility of recombinant proteins in E. coli and to provide efficient protein purification coupled with tag removal.
The Pressure Cycling Technology Sample Preparation System (“PCT SPS”) employs rapid cycles of hydrostatic pressure, between ambient and ultra high levels, to precisely control biomolecular interactions. The PCT SPS can be used to accelerate enzymatic reactions, such as protein digestion with trypsin and other proteolytic enzymes, to prepare samples for analysis by mass spectrometry.
Ingenio™ Electroporation Solution – The High Efficiency, Broad Spectrum Solution For Nucleic Acid Delivery into Primary Cells and Hard to Transfect Cell-lines
Primary cells as well as certain cell-lines are known to be refractory to traditional chemical transfection methods. The physical technique of electroporation has emerged as a method of choice for nucleic acid delivery into these “hard to transfect” cells.
Cell and Tissue Culture-Human Pathogen/Mycoplasma Testing
Detection and Prevalence of HIV, Hepatitis B and Mycoplasma sp. in Human Cell Lines and Tumors with h-IMPACT Assays
Human cell lines and tumors represent important tools in biomedical research and they are extensively used. The most common sources of human cell lines and tumors are specimens taken directly from patients and cultured specimens stored and distributed by repositories and cell banks.
Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and has a substantial negative impact on biomedical research. In this study, an approach used by RADIL for analyzing human and rodent cell lines for inter and intra--species contamination followed by authentication of cell line identity is described.