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POSTER
Plasmid Purification Poster
Sponsored by
Pall Life Sciences
Published in
December 2010
2010
(p.11)
DOI: 10.2144/000113599
Sponsored,vendor-submitted protocol
Advances in cloning techniques have greatly increased the number of samples
requiring small-scale plasmid preparation, or mini preps. The large scale
purification schemes of the past have given way to the development of small
scale, massively parallel purifications requiring semi-automated or
fully-automated handling. Pall has created a cost effective alternative for
efficient parallel lysate clarification and plasmid DNA purification from E.
coli cultures. The process starts with an optimized lysate clarification
filter plate followed by purification on a high binding capacity DNA binding
filter plate. Filtration can be done effectively in either a vacuum or
centrifugal mode, ultimately maximizing the choice in protocols available to
the researcher. In this poster, we compare vacuum filtration to
centrifugation for DNA purification. In addition, we demonstrate the benefit
of using a filter plate configuration with an integral prefilter for lysate
clarification.
More Information
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Pall Life Sciences
Contact Information
600 South Wagner Road
Ann Arbor
MI
48103-9019
USA
Tel:734-665-0651
Fax:734-913-6114
E-mail:
LabSupport@Pall.com
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