to BioTechniques free email alert service to receive content updates.

Plasmid Purification Poster
Sponsored by Pall Life Sciences Published in December 2010 2010 (p.11) DOI:  10.2144/000113599 Sponsored,vendor-submitted protocol
Advances in cloning techniques have greatly increased the number of samples requiring small-scale plasmid preparation, or mini preps. The large scale purification schemes of the past have given way to the development of small scale, massively parallel purifications requiring semi-automated or fully-automated handling. Pall has created a cost effective alternative for efficient parallel lysate clarification and plasmid DNA purification from E. coli cultures. The process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter plate. Filtration can be done effectively in either a vacuum or centrifugal mode, ultimately maximizing the choice in protocols available to the researcher. In this poster, we compare vacuum filtration to centrifugation for DNA purification. In addition, we demonstrate the benefit of using a filter plate configuration with an integral prefilter for lysate clarification.
More Information
Do you have question about this Poster? Would you like more information about this poster?
Submit your questions here and BioTechniques will forward your question and contact information from our database to an expert at Pall Life Sciences
Request more information
Yes, please forward my question, along with my name, title, company, and e-mail address to Pall Life Sciences
BioTechniques will forward your question to the company, who will contact you directly by email.
Name Question
Job Title
Contact Information
Poster Categories