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POSTER
Streamlined Purification of Plasmid DNA from Prokaryotic Cultures
Sponsored by
Pall Life Sciences
Published in
September 2010
2010
(p.10)
DOI: 10.2144/000113525
Sponsored,vendor-submitted protocol
Advances in cloning techniques have greatly increased the number of samples requiring small-scale plasmid preparation, or mini preps. The large scale purification schemes of the past have given way to the development of small scale, massively parallel purifications requiring semi-automated or fully-automated handling. Pall has created a cost effective alternative for efficient parallel lysate clarification and plasmid DNA purification from E. coli cultures. The process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter plate. Filtration can be done effectively in either a vacuum or centrifugal mode, ultimately maximizing the choice in protocols available to the researcher. In this poster, we compare vacuum filtration to centrifugation for DNA purification. In addition, we demonstrate the benefit of using a filter plate configuration with an integral prefilter for lysate clarification.
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Pall Life Sciences
Contact Information
600 South Wagner Road
Ann Arbor
MI
48103-9019
USA
Tel:734-665-0651
Fax:734-913-6114
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