Poster Hall 2011
HisTrap™ FF crude is a ready to use column, intended for purification of histidine-tagged proteins from unclarified cell lysates. No centrifugation or filtration of the samples is required.
The Strep-tag II is ideal as an affinity tag due to the small size (8 aa, 1 kDa), often making removal of the tag unnecessary. StrepTrap™ HP 1 ml and 5 ml columns have been specially developed for high specificity towards the Strep-tag II, with the prepacked format facilitating fast and convenient purification.
HisTrap FF crude, is developed for fast, simple and convenient purification of histidine-tagged proteins. Unclarified cell lysate can be applied directly on the column, without any centrifugation and filtration steps.
Gel filtration (GF) is an excellent tool to acquire information about sizing (identity), purity and specially the multimeric state of a protein of interest. Superdex™ 200 and Superdex 75 are supreme GF media for such analysis.
Maltose binding protein (MBP) is beneficial as an affinity tag due to its ability to increase expression level and solubility of the fusion protein. The MBPTrap™ HP 1 ml and 5 ml columns have been specially developed for high specificity towards the MBP-tag and the prepacked format facilitate fast, convenient and reproducible purifications.
qPCR Efficiency Calculations