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Rapid protein analysis and screening of membrane protein buffer conditions by gel filtration
Inger Salomonson1 and Said Eshaghi2
1GE Healthcare Bio-Sciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden
2Karolinska Institute, Stockholm, Sweden
Sponsored by GE Healthcare
Published in July 2011 (p.18) Sponsored,vendor-submitted protocol
Gel filtration (GF) is an excellent tool to acquire information about sizing (identity), purity and specially the multimeric state of a protein of interest. Superdex™ 200 and Superdex 75 are supreme GF media for such analysis. To speed up analysis and keep sample and buffer consumption at minimum, two prepacked short GF columns have been developed, Superdex™ 200 5/150 GL and Superdex 75 5/150 GL. With lengths of only 15cm and volumes of 3ml, these columns allow rapid analysis (6-12min/run) with minimal sample (4-50µl) and buffer consumption.
Results from different applications such as protein-protein interaction, rapid screening of buffer conditions for membrane proteins and fast purity check of a histidine-tagged protein using short gel filtration columns will be presented.
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