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Zafer Bashi, George Khachatourians, Dwayne Hegedus
Fungal hyphae—and in some cases, spores—are multi-nucleate. During genetic transformation of these spores or mycelia, only one nucleus generally receives the transferred T-DNA...
Jeffrey M. Perkel
1 )—they also make for lousy screens. Those drugs currently on the market are the fruits of serendipity, not high-throughput screening (HTS), says Michael Dabrowski, head of AstraZeneca's...
Nathan Blow, Kristie Nybo
Delivering plasmid DNA into cells to create mutant lines is a common and well-established procedure for bacteria, plant, and animal cells. However, this procedure becomes complicated when it comes to...
Nijsje Dorman, Patrick C.H. Lo, Kristie Nybo
Just as nanofluidics has stolen some of the limelight from micro-fluidics, advances in nanoscopy are beginning to make standard microscopy seem a bit passe. One of the first techniques described for...
Cheul H. Cho, Jaesung Park, Arno W. Tilles, François Berthiaume, Mehmet Toner, Martin L. Yarmush
Microfabrication and micropatterning techniques in tissue engineering offer great potential for creating and controlling microenvironments in which cell behavior can be observed. Here we present a...
Kevin Ahern
It doesn't take an electron microscope to produce beautiful and educational images of tiny life forms. That message is clearly in focus at Micrographia.com, where an eclectic collection of (mostly)...
can't eventually automate?
Crystallography has several steps where, in the past, experimental design and setup had been the realm of the specialist. Generating diffraction-quality crystals takes...
Emmanuel Vanrobays*, Barbara H. Jennings**, David Ish-Horowicz
One of the many advantages of Drosophila melanogaster as a model organism is the relative ease with which gene deletions can be generated by imprecise excision of transposon insertions. Here, we...
Kristie Nybo
This month's question from the Molecular Biology Forums (online at molecularbiology. forums.biotechniques.com) comes from the “Real-Time qPCR/qRT-PCR Methods” section. Entries have been edited for...
Kristie Nybo
Jeff Doyle's pioneering work in the field of plant phylogenetic systematics caught our attention. Curious to know more, BioTechniques contacted him to find out about the ambitions, character,...
Hernan Lorenzi, Nichole Duvall, Sheila M. Cherry, Roger H. Reeves, Randall J. Roper
Down syndrome (trisomy 21, or DS) is the most common live-born aneuploidy in humans, occurring in approximately 1 in 700 live births
