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Quantification of DNase type I ends, DNase type II ends, and modified bases using fluorescently labeled ddUTP, terminal deoxynucleotidyl transferase, and formamidopyrimidine-DNA glycosylase
David S. Baskin, Marsha A. Widmayer, Martyn A. Sharpe
Here we describe the substitution of fluorescently labeled ddUTP for dUTP in the TUNEL assay to allow quantification of generated fluorescence signals by epifluorescence microscopy. The capping of...
The Method—Not the Meaning—of Life
1 ), blogs and news outlets alike were buzzing about this new form of “synthetic life.” The media furor that resulted quickly incited questions about the future of synthetic biology, its moral...
Returning to Diagnostic Basics
Sarah A. Webb
Shuffling cards In the late 1990s, the development of soft lithography methods using polydimethylsiloxane (PDMS) by George Whitesides' group at Harvard University and others brought microfluidics...
BioSpotlight
Patrick C.H. Lo, Kristie Nybo
Certain recombinant fusion proteins require short peptide linkers between protein domains to provide flexibility or increase stability during synthesis. For some fusion proteins, however, the...
Citations
Nijsje Dorman, Patrick C.H. Lo, Kristie Nybo
Small fluorescent labels such as the fluorescein arsenical hairpin binder (FlAsH) make popular alternatives to bulky fluorescent proteins. FlAsH, along with its color variant ReAsH (red arsenical...
Miniaturized FISH for screening of onco-hematological malignancies
Andrea Zanardi, Dario Bandiera, Francesco Bertolini, Chiara Corsini, Giuliana Gregato, Paolo Milani, Emanuele Barborini, Roberta Carbone
Fluorescence in situ hybridization (FISH) represents a major step in the analysis of chromosomal aberrations in cancer. It allows the precise detection of specific rearrangements, both for diagnostic...
Immunology and Immunochemistry: Fluorescence Activated Cell Sorting (FACS)
Kristie Nybo
This month's question from the Molecular Biology Forums (online at
The nuclear translocation assay for intracellular protein-protein interactions and its application to the Bcr coiled-coil domain
Andrew S. Dixon, Carol S. Lim
Protein interactions are critical for normal biological processes and molecular pathogenesis. While it is important to study these interactions, there are limited assays that are performed inside the...
Design of an in vivo cleavable disulfide linker in recombinant fusion proteins
Xiaoying Chen, Yun Bai, Jennica L. Zaro, Wei-Chiang Shen
In order to achieve optimal biological activity and desired pharmacokinetic profiles, a dithiocyclopeptide linker was designed for in vivo release of protein domains from a recombinant fusion...
Profile of Jeff Lichtman
Kristie Nybo
Jeff Lichtman's research on neuronal development and his role in creating the Brainbow mouse caught our attention </div> </div> <br clear="all" /> <div class="backtoTop"> <a href="#">Back to top</a> <a class="backtoImg" href="#"> <img height="14" width="14" border="0" src="/template/ver1-0/i//backtotop.gif" /> </a> </div> </div> </form> <br clear="all" /> <div class="btn_middleAdv"> <script> <!-- OAS_AD('Middle'); //--> </script> </div> <br clear="all" /> <!--Bottom Advertising End here--> <!-- start more from biotechniques --> <div id="box" align="center"> <div class="spiffyfg" align="left"> <div class="bottompanel_reform"> <div class="heading3">More from BioTechniques:</div> <div class="moreAuthors"> <div class="panel_heading"> For Authors</div> <div class="moreBioImg"> <img border="0" src='http://www.biotechniques.com/multimedia/archive/00009/forauthors_9153a.gif'/> </div> <div class="paneltxt1"> <a href="/morefrombiotechniques/forauthors/submitpapers/">Submit Papers</a> </div> <div class="paneltxt1"> <a href="/morefrombiotechniques/forauthors/submitcovers/">Submit Covers</a> </div> <div class="paneltxt1"> <a href="/morefrombiotechniques/forauthors/orderreprints/">Order Reprints</a> </div> </div> <div class="moreAdvetisers"> <div class="panel_heading"> For Advertisers</div> <div class="moreBioImg"> <img border="0" src='http://www.biotechniques.com/multimedia/archive/00009/foradvertisers_9154a.gif'/> </div> <div class="paneltxt2"> <a href="/morefrombiotechniques/foradvertisers/advertise_with_biotechniques/">Advertise</a> </div> <div class="paneltxt2"> <a target="_blank" href="http://www.biotechniques.com/multimedia/archive/00009/Media_Kits_9156a.pdf">Media Kits</a> <a href="/morefrombiotechniques/foradvertisers/circulationstatements/">Circulation Statements</a> </div> <div class="paneltxt2"> <a href="/morefrombiotechniques/foradvertisers/ListRental/">List Rental</a> </div> </div> <div class="moreSubscribers"> <div class="panel_heading"> For Subscribers</div> <div class="moreBioImg"> <img border="0" src='http://www.biotechniques.com/multimedia/archive/00009/forsubscribers_9155a.gif'/> </div> <div class="paneltxt3"> <!-- added for 2358 --> <a href="/email/">Change Address/email</a> </div> <div class="paneltxt3"> <!-- added for 2358 --> <a href="/email/">Manage Subscriptions</a> </div> <div class="paneltxt3"> <!-- added for 2358 --> <a href="http://link.argifocus.com/0E4-66">Request Free Info</a> </div> <div class="paneltxt3"> <!-- added for 2358 --> <a href="http://www.biotechniques.com/morefrombiotechniques/forsubscribers/permissions/">Permissions</a> </div> </div> </div> </div> </div> <!-- End more from biotechniques --> </div> <div id="btn_RRWraper"> <!-- Start email-alerts section --> <div style="float:left;margin-top:1px;"> <script language="javascript"> <!-- OAS_AD('TopRight'); //--> </script> <div style="margin-top:5px;"> <script language="javascript"> <!-- OAS_AD('Right'); //--> </script> </div> </div> <!-- End of right container --> <!-- End email alerts --> </div> </div> </div> <script language="javascript"> function validateSearchForm(section) { if (document.getElementById("key").value == "") { alert("Please enter keyword"); return false; } else if (document.getElementById("key").value.length < 3) { alert("Search keyword should be atleast 3 character long."); return false; } else if (document.getElementById("key").value.charAt(0) == ' ') { alert("Search keyword should not start with space."); return false; }else if (checkForSpecialChar(document.getElementById("key").value)) { alert("Special characters are not allowed in Search keyword."); return false; } else { // document.getElementById("searchId").href=section+"search.do?pageNumber=1§ionName=Home&key="+document.getElementById("key").value; document.footerSearchForm.action = section + "search.do?pageNumber=1§ionName=Home&key=" + document.getElementById("key").value; document.footerSearchForm.submit(); } } function checkForSpecialChar(fieldValue) { //field=value var iChars = "!@#$%^&*()+=-[]\\\';,./{}|\":<>?"; var flag=false; for (var i = 0; i < fieldValue.length; i++) { if (iChars.indexOf(fieldValue.charAt(i)) != -1) { // alert ("Special characters are not allowed in "+fieldName + " field"); flag=true; break; } } return flag; } </script> <script type="text/javascript"> var gaJsHost = (("https:" == document.location.protocol) ? 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