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Protein arrays are becoming ubiquitous in research laboratories worldwide, as they provide a simple and high-throughput solution for proteomics research. Although commercial arrays are now becoming...
Alexios N. Polidoros, Konstantinos Pasentsis, Athanasios S. Tsaftaris
1 , 2 ) numerous modifications and improvements of the method have been developed and consist of a collection of PCR-based cloning procedures that extend a known cDNA fragment toward the 3′...
Alexios N. Polidoros, Konstantinos Pasentsis, Athanasios S. Tsaftaris
The publication of the human and other metazoan genome sequences has greatly facilitated positioning and analysis of various genomic functional elements, with initial emphasis on coding sequences....
Cesar E. Guerra
A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for...
David D. Smith, Susan Kovats, Terry D. Lee, Leticia Cano
We constructed protein arrays according to a titration design to estimate the assay sensitivities over varying concentrations of flu vaccine and human immuno globulin G (IgG). After imaging, we...
Daqian Sun, Margherita Melegari, Sunandini Sridhar, Charles E. Rogler, Liang Zhu
A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be...
Since the discovery that, with careful attention and manipulation, stem cells could be maintained in culture, one of the holy grails of transgenic research has been the ability to use spermatogonial...
Lynne Lederman
Two years ago, the first Technology News column citation reported that nanotechnology would probably continue to be best thought of as a collection of technologies capable of providing solutions to a...
Xu-Gang Xia, Hongxia Zhou, Zuoshang Xu
RNA interference (RNAi) has been increasingly used for reverse genetics. Both pol III and pol II promoters have been used to synthesize short hairpin RNA (shRNA) for knockdown of gene expression in...
Jinke Wang, Min L. Li, Dong Hua, Qixin Chen
This paper describes an exonuclease-mediated enzyme-linked immunosorbent assay (ELISA)-like assay (EMEA) for detecting the DNA binding activity of nuclear factor κB (NF-κB). For EMEA, a...
Igor P. Chernov, Sergey B. Akopov, Lev G. Nikolaev, Eugene D. Sverdlov
Specific binding of nuclear proteins, in particular transcription factors, to target DNA sequences is a major mechanism of genome functioning and gene expression regulation in eukaryotes. Therefore,...
Masayoshi Itoh, Ayako Yasunishi, Kengo Imamura, Mutsumi Kanamori-Katayama, Harukazu Suzuki, Masanori Suzuki, Piero Carninci, Jun Kawai, Yoshihide Hayashizaki
The Return of the Native Some of the most widely used ORF resource clones dispense with native termination codons. This makes perfect sense if the goal is to permit rapid exchange...
Joel R. McNeal, James H. Leebens-Mack, Kathiravetpillai Arumuganathan, Jennifer V. Kuehl, Jeffrey L. Boore, Claude W. dePamphilis
Organellar genome sequences provide numerous phylogenetic markers andyield insight into organellar function and molecular evolution. These genomes are much smaller in size than their nuclear...
Mary McCarthy, Nijsje Dorman, Sean Sanders
For more than 23 years, BioTechniques has been a familiar fixture on lab benches everywhere—as fundamental and indispensable a tool as the pipettor and graduated cylinder. This month, the...
