ATCC's STR Profiling Service for human cell line authentication is designed to be an easy and sure solution to verify the identity of their human cell lines.
Researchers simply apply cells to an FTA card following the instructions
provided with the FTA Sample Collection Kit. The need to ship samples on dry
ice is eliminated. ATCC will amplify the DNA using the Promega PowerPlex 16
HS System and analyze the results. Investigators can expect results via
e-mail within 5 days from receipt of their sample at ATCC.
ATCC offers two levels of service: Basic and Verified. The Basic STR Profiling
Service (135-X) includes a signed Cell Line Authentication Report containing
an easy-to-understand STR allele table and electropherograms that support
the allele calls at each locus. The Verified STR Profiling Service (135-XV)
includes everything in the Basic service, plus known reference profiling
against the ATCC STR Profile database, and a comprehensive interpretation of
Unlike normal diploid cells, many continuous cell lines – especially cancer
cell lines – are karyotypically abnormal or genetically unstable, making
data interpretation difficult. And as misidentified cell lines continue to
plague research, human cell line authentication via STR analysis is becoming
a requirement of many journals prior to publication, and funding agencies.
STR loci are among the most informative polymorphic markers in the genome.
ATCC generates the STR profile by simultaneously amplifying 15 STR loci,
plus the amelogenin gene for gender determination, in a multiplex PCR
reaction enabling a 1 in >1017 matching rate for unrelated individuals.
Since 1925, ATCC has operated as an independent, non-profit entity that has
helped life science organizations and academic institutions produce
innovations that have benefited humankind: creating life saving vaccines,
nutritional improvements, cancer research breakthroughs, and safer, more
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working relationship with 12 distribution partners, ATCC has the experience,
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