NuGEN’s Applause 3’-Amplification System offers a single-tube solution for routine microarray sample preparation.
NuGEN’s Applause 3’ amplification system offers a single-tube solution for
routine microarray sample preparation. Starting at sample sizes of 50 ng,
the system readies RNA samples in six hours for microarray gene expression
analysis using its linear Ribo-SPIA (Single Primer Isothermal Amplification)
The system is designed for use with Affymetrix GeneChip 3’ expression, Gene
1.0 ST, and Exon 1.0 ST arrays. These add-and-incubate assays increase
sensitivity and produce results faithful to the transcriptome. The system
includes all reagents needed to generate micrograms of material from RNA
samples with a simple workflow. Requiring only a single purification step,
targets are ready for hybridization in one day.
Current methods for generating targets for GeneChip 3’ expression, Exon 1.0
ST, and Gene 1.0 ST arrays require multiple purification steps and upwards
of 24 hours of preparation time.
NuGEN Technologies, Inc., based in San Carlos, CA, is focused on the
development and commercialization of sensitive, rapid, and high-throughput
amplification and sample preparation systems to enable the comprehensive
analysis and discovery of biological mechanisms, cellular responses, and