to BioTechniques free email alert service to receive content updates.
Molecular Biology
Bisulfite Conversion System
Promega's MethylEdge Bisulfite Conversion System is designed to offer an efficient method for rapidly performing bisulfite conversion and DNA clean-up within two hours, while maintaining DNA integrity to provide more intact, converted DNA for a variety of sensitive applications.
The system requires only small amounts of input genomic DNA (100pg-2µg). Using
optimized conversion reagents, clean-up, and recovery methods, the system
converts DNA at ≥90% efficiency with minimal fragmentation. The resulting
DNA is suitable for use in downstream applications such as PCR, array, or
sequencing assays. The kit includes all reagents necessary to perform 50
bisulfite conversion reactions. All reagents can be stored at room
temperature and require minimal up-front preparation. Control DNAs are also
available separately and can be used as standards in both the conversion
reaction and post-conversion analysis.
