The Leica DFC365 FX digital microscope camera is designed to document live cells and rapidly fading fluorescence specimens.
The system is equipped with a sensitive CCD sensor and active Peltier cooling
for a wide range of applications, including basic fluorescence
documentation, TIRF, FRET, and structured illumination. The camera achieves
acquisition rates of 21 fps at full resolution. In its overlapping mode, an
image can be captured while the previous image is still being read out. Data
are transferred to the PC via a FireWire-B interface. In addition to a 40
MHz acquisition speed, the pixel clocking rate of the sensor can also be set
to 20 MHz or 1.6 MHz as required. The optional NIR mode extends the
operating range of the camera for fluorescence markers emitting in the
wavelength range above 700 nm, which are difficult to capture with
conventional CCD technology.