Integrated DNA Technologies' double-quenched probes are designed to increase the accuracy and reliability of 5’ nuclease qPCR experiments.
The probe design positions an internal ZEN quencher 9 bases from the 5’
fluorophore, which when combined with the standard 3’ quencher,
significantly decreases background fluorescence and increases sensitivity.
Due to the incorporation of ZEN, the initial background fluorescence signal
is much lower, making subsequent changes easily detectable while assay
sensitivity is increased. As a result low background is obtained, even at 40
base pairs or longer, increasing specificity without loss in sensitivity or
quenching efficiency. This functionality increases qPCR accuracy and
sensitivity when compared to traditional probes.
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic
acids in the United States, serving academic, government, and commercial
researchers in biotechnology, clinical diagnostics, and pharmaceutical
development. IDT's primary business is the manufacture of custom, synthetic
DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of
36,000 custom oligos per day to more than 86,000 customers worldwide. IDT
manufacturing locations include facilities in Coralville, Iowa; San Diego,
Calif.; and Leuven, Belgium.