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Cell Culture
Electric Fence Cell Migration Assay
Applied BioPhysics’ "electric fence" assay is designed to measure the rate of cell migration in tissue culture.
The technique is based on the company’s Electric Cell-substrate Impedance
Sensing (ECIS) technology, an impedance-based method to monitor cell
behavior. In the assay, high current pulses are applied to the small ECIS
electrode immediately after cell inoculation to prevent cells from attaching
and spreading, while a cell layer forms on the rest of the substrate. When
the “electric fence” is turned off, cells surrounding the open electrode
migrate in to fill the void. The progress of this migration is electrically
monitored and a migration rate is calculated. The “electric fence” assays
are easy-to-do and make an attractive replacement to the mechanical
scrape-wound approach. Published applications using ECIS include
measurements of cell migration, endothelial barrier function, extravasation
of normal cell layers by metastatic cells, signal transduction, cell-ECM
interactions, cytotoxicity, cytopathic effects of viral infections and cell
proliferation.
This Product is made by:
Applied BioPhysics,Inc.is focused on applying the results of biophysical research to provide practical tools for cell research and drug discovery.
