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Nucleic-acid technologies: PCR
EpiQ chromatin analysis kit
Bio-Rad's new EpiQ chromatin analysis kit is a real-time PCR assay for the rapid quantitative assessment of chromatin structure.
Complementing existing epigenetic assays such as DNA methylation and chromatin immunoprecipitation, the EpiQ kit is the first commercial research tool that helps scientists quantify the impact of epigenetic events on gene expression regulation through chromatin state changes. The EpiQ kit can provide quantitative information about chromatin accessibility, which correlates very strongly with gene expression. Until now, the only way to quantitatively probe the status of chromatin structure was through the use of homebrew methods, first described in 2001 (Rao, et. al., J. Immunol.). This approach requires the isolation and purification of nuclei from several millions cells prior to chromatin digestion and takes more than two days to complete. With the EpiQ kit, chromatin structure data can be obtained within six hours from as few as 50,000 cultured cells, without the need for nuclei isolation. The chromatin state of a gene can be identified in situ based on its sensitivity to the action of the nuclease in the EpiQ kit. In heterochromatin, genomic DNA is inaccessible to nuclease digestion and remains available for subsequent qPCR. Analysis of heterochromatin using the EpiQ kit reveals a minimal quantification cycle (Cq) shift between digested and undigested samples. In contrast, DNA in euchromatin is susceptible to nuclease digestion and is unavailable for qPCR. Analysis of euchromatin using the EpiQ kit shows a large Cq shift between digested and undigested samples. EpiQ kit components include buffers for cell permeabilization and in situ chromatin digestion, optimized nuclease, materials for genomic DNA purification, control assays (qPCR primers) for chromatin assessment of a reference (epigenetically silenced) and control (constitutively expressed) gene, and EpiQ Chromatin SYBR® Green Supermix, a real-time PCR reagent designed to amplify genomic DNA. The supermix can be used on all Bio-Rad real-time PCR instruments and on those from other suppliers; some instruments may require slight modifications to the supermix.
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Bio-Rad Laboratories is a corporation providing a broad range of innovative tools and services to the life science research and clinical diagnostics markets. Founded in 1952, Bio-Rad has a global team of more than 6,500 employees and serves more than 85,000 research and industry customers worldwide through its global network of operations. Throughout its existence, Bio-Rad has built strong customer relationships that advance scientific research and development efforts and support the introduction of new technology used in the growing fields of genomics, proteomics, drug discovery, food safety, medical diagnostics, and more.