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Molecular Biology
Genome-wide qPCR Primers for Use with Intercalating Dyes
Integrated DNA Technologies (IDT) genome-wide PrimeTime qPCR Primers are designed for use with SYBR Green, EvaGreen, and other intercalating dyes in qPCR assays.
The PrimeTime qPCR Primers are the same primer pairs used in the renowned PrimeTime qPCR Assays, selected to detect and quantify gene expression in human, mouse, and rat transcriptomes. The shipped product consists of a pair of pre-mixed primers, normalized to 5 nmoles, supplying researchers with enough primer for 500 20-µL qPCR reactions with an efficiency of more than 90%. Pre-designed and synthesised at the time of order, each set of primers incorporates the most up-to-date sequence information, along with accurate melting temperature (Tm) and secondary structure predictions. Each is BLAST searched to eliminate off-target effects and to avoid the impact of single nucleotide polymorphisms (SNPs).
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Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium.