Integrated DNA Technologies (IDT) genome-wide PrimeTime qPCR Primers are designed for use with SYBR Green, EvaGreen, and other intercalating dyes in qPCR assays.
The PrimeTime qPCR Primers are the same primer pairs used in the renowned
PrimeTime qPCR Assays, selected to detect and quantify gene expression in
human, mouse, and rat transcriptomes. The shipped product consists of a pair
of pre-mixed primers, normalized to 5 nmoles, supplying researchers with
enough primer for 500 20-µL qPCR reactions with an efficiency of more than
90%. Pre-designed and synthesised at the time of order, each set of primers
incorporates the most up-to-date sequence information, along with accurate
melting temperature (Tm) and secondary structure predictions. Each is BLAST
searched to eliminate off-target effects and to avoid the impact of single
nucleotide polymorphisms (SNPs).
This Product is made by:
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic
acids in the United States, serving academic, government, and commercial
researchers in biotechnology, clinical diagnostics, and pharmaceutical
development. IDT's primary business is the manufacture of custom, synthetic
DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of
36,000 custom oligos per day to more than 86,000 customers worldwide. IDT
manufacturing locations include facilities in Coralville, Iowa; San Diego,
Calif.; and Leuven, Belgium.