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Nucleic-acid technologies: PCR
Genome-wide qPCR Primers
The PrimeTime qPCR Primers are the same primer pairs used in the renowned
PrimeTime qPCR Assays, selected to detect and quantify gene expression in
human, mouse, and rat transcriptomes. The shipped product consists of a
pair of pre-mixed primers, normalized to 5 nmoles, supplying researchers
with enough primer for 500 20-µL qPCR reactions with an efficiency of more
than 90%.
Pre-designed and synthesized at the time of order, each set of primers
incorporates the most up-to-date sequence information, along with accurate
melting temperature (Tm) and secondary structure predictions. Each is BLAST
searched to eliminate off-target effects and to avoid the impact of single
nucleotide polymorphisms (SNPs).
Since the same primer pairs are used across the PrimeTime qPCR Primers and
PrimeTime qPCR Assays, the transition from discovery to screening is easy.
This Product is made by:
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium.
