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Nucleic-acid technologies: PCR
GoTaq 2-Step RT-qRCR System
The Promega GoTaq 2-Step RT-qPCR System allows researchers to detect target genes expressed at low levels even with hard-to-amplify samples.
Enhanced sensitivity of the two-step system extends the broad dynamic range of RNA quantitation and provides a robust gene expression analysis tool. GoTaq 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript Reverse Transcription System. Optimized reagents and ultra-active, GoScript Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. The transcription system improves the yield of cDNA even in the presence of common inhibitors such as ethanol. Promega GoTaq 2-Step RT-qPCR provides senstivve detection down to 1fg across an extended linear range of RNA input. In the second step, cDNA is quantified using the GoTaq qPCR Master Mix. Formulated with a hot-start DNA polymerase, the GoTaq qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq qPCR Master Mix contains BRYT GREEN dye which can be twice as bright as of SYBR Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges. The GoTaq 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript Reverse Transcription System with the ultra-bright fluorescence of GoTaq qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.
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Promega is a leader in providing innovative solutions and technical support to the life sciences industry. Promega Corporation’s 2,000 products enable scientists worldwide to advance their knowledge in life science research, particularly in genomics, proteomics, and cellular analysis. Our products are also used to support molecular diagnostics and human identification. Founded in 1978, Promega Corporation is headquartered in Madison, Wisconsin, USA, with branches in twelve countries and over 50 global distributors.